卵叶韭休眠芽中GA_3I、AA、ABA和ZT的高效液相色谱法测定

Determination of GA_3,IAA,ABA and ZT in Dormant Buds of Allium ovalifolium by HPLC

以卵叶韭休眠芽为试材,改进了提取纯化赤霉素(GA3)、生长素(IAA)、脱落酸(ABA)和玉米素(ZT)4种内源激素的处理方法和色谱条件。采用Hypersil ODS C18柱(150 mm×4.6 mm,5μm),甲醇-0.6%冰乙酸进行梯度洗脱,紫外检测波长254 nm。实际分析样品表明,本法分离度好,准确度高,适合大批量测定。

An improved method of extraction, purification and determination to four internal hormones （GA3, IAA, ABA and ZT） in dormant buds of Allium ovalifolium with Reversed-phase High Performance Liquid Chromatography has been developed. Chromatographic conditions are： Hypersil ODS C18 reverse phase column （ 150 mm× 4.6 mm, 5μm）, methanol - 0.6 % acetic acid mobile phase, gradient elution, UV detector set at 254 nm. The result shows that this methods is a accurate and high-separation-efficiency technique, suitable to determine large quantities of samples.