农药残留的酶联免疫分析技术及研究进展

Technology of Immunoassay of ELISA for Pesticide Residues and Its Research Progress

免疫分析方法,尤其是酶联免疫吸附分析法,灵敏度高,特异性强,操作简便快捷,在现场筛选和大量样本的快速检测中显示了其独特的优势。为此,介绍了农药残留酶联免疫分析技术的原理、方法建立中半抗原的制备、人工抗原的合成、抗体的制备等核心技术和酶联免疫分析技术在农药残留快速监测中的应用进展,并讨论了对农药残留的酶联免疫分析技术存在的问题和应用前景。

Immunoassay（IA）,especially ELISA with high sensitivity ,the strong particularity, simple and rapid when being manipulated, and these show its unique superiority in field analysis and sample rapid monitoring .In this paper, the preparation of hatpin synthesis, the synthesis of hatpin protein conjugation, the preparation of antibody in the foundation of principal and method of ELISA of pesticide residues as well as the application of ELISA in rapid monitoring of pesticide residues, were introduced and some questions and the new perspective of immunoassay of ELISA for prospective of immunoassay of ELISA for pesticide residues were also discussed.