泗棉3号理想株型的遗传及分子标记研究

Inheritance and QTLs Tagging for Ideal Plant Architecture of Simian 3 Using Molecular Markers

构建了泗棉3号×CARMEN组合的RIL群体,研究棉花高产株型性状的遗传规律和分子机理。对该群体的株型性状进行遗传模型分析,结果表明,这些性状的最适模型都是主基因+多基因遗传模型,说明存在控制这些性状的主基因,同时,株型性状是以多基因遗传为主。用2523对SSR引物,进行双亲的多态性检测,有62对SSR引物具有多态性,产生65个稳定的多态性位点。利用复合区间作图法进行QTL检测,共检测到5个稳定可靠的株型性状的QTLs,为3个株高/果枝长度比的QTLs,以及株高、果枝长度的QTLs各1个,这些QTLs都是在2个环境中或以2个环境平均数检测到的。单标记分析,还检测到9个稳定可靠的株型性状的QTLs。检测到的QTLs和筛选到的分子标记,可以用于对株型性状的MAS。

A RIL population of Simian 3 × CARMEN had been developed to study the genetics and tag the QTLs controlling the ideal plant architecture traits of high yield. Jointly segregating analysis of P1, P2 generations and RIL population was used to analyze the genetics of plant architecture traits in （Simian 3XCARMEN） by using the method of major gene plus polygene mixed inheritance model. The optimum models of all traits are major gene plus polygene mixed inheritance model. This indicated that the inheritance of the traits was controlled by major genes. QTL tagging of plant architecture traits in （Simian 3 X CARMEN ） RIL population was conducted with 65 SSR markers having polymorphism between parents with screened from a total of 2523 SSR primers pairs. The results showed that 5 QTLs controlling traits about plant architecture had been tagged in two environments or by using the mean of lines in two environments based on composite interval mapping and marker multiple regression methods. Simultaneously, 9 QTLs controlling plant architecture traits had been detected u- sing the single-marker analysis in two environments or the mean of lines in two environments and at least in one environment. The molecular markers tightly linked with the QTLs can be used for the MAS of ideal plant architecture in high-yield breeding program.