丹参药材脂溶性成分的HPLC指纹图谱

Studie on the fingerprint of Radix et Rhizoma Salviae Miltiorrhizae

目的建立丹参药材的高效液相指纹图谱分析方法。方法采用Kromasil C18（4．6mm×250mm,5μm）色谱柱，以体积分数为0．02％磷酸乙腈（A）与体积分数为0．02％磷酸水（B）梯度洗脱。线性梯度洗脱程序为：0～15min，20％～30％A；15～18min，30％～50％A；18～35min，50％～60％A；35～60min，60％～80％A；60～75min，80％～100％A；75～100min，100％A。流速为1．0mL·min^-1，柱温为40℃，检测波长280nm，以丹参酮ⅡA为参照物。结果通过对11批不同产地丹参药材的测定，标定了14个共有峰，并通过“中药指纹图谱计算机辅助相似度软件”，计算出其相似度均在0．9以上。结论该方法准确可靠，重现性好，为更好地控制丹参内在质量提供了科学依据。

Objective To establish the method of fingerprint analysis of Radix et Rhizoma Salviae Miltiorrhizae by HPLC. Methods Kromasil C18（4.6 mm× 250 mm, 5 μm）column was used. The mobile phase was composed of 0.02% H3PO4-CH3CN and 0.02 % H3PO4-H2O with a linear gradient elution. The flow rate was 1.0 mL·min^-1 with DAD detected at 280 nm. The column temperature was maintained at 40℃. Results The 14 common peaks were pinpointed by the determination of the 11 Radix et Rhizoma Salviae Mildorrhizae samples of different specifications gathered from different places. The similarities, which were proved to be higher than 0.9, were calculated with the help of The Similarity Calculation Soft of Fingerprint Chromatography of Traditional Chinese Medicine. Conclusions This method is accurate, reliable and provides a scientific basis for controlling the quality of Radix et Rhizoma Salviae Miltiorrhizae.