提高马铃薯双单倍体花药培养产生胚状体及再生植株频率的研究

STUDY ON INCREASING FREQUENCY OF EMBROIDS AND REGENERATED SHOOTS FROM DIHAPLOID POTATO BY ANTHER CULTURE IN VITRO

用三种不同温度前处理和三种培养基相配合对７个从双单倍体马铃薯（Ｓ．ｔｕｂｅｒａｓｕｍＬ，２ｎ＝２ｘ＝２４）中随机抽取的品系进行了花药培养，选择出了较好的培养基和前处理的适宜温度及方法．从双单倍体花药中诱导产生胚状体及再生植株频率有所提高达２７．５％和８．８％．倍性鉴定表明，从双单倍体诱导的胚状体分化的植株绝大多数为二倍体，而且多数为纯合体，说明这些胚状体来自己减数的配子，可能是由在胚状体发育过程中的体细胞染色体加倍形成的．

Authers from 7 clones of dihaploids of potato (S. tuberosum L.) were cultured on 5 different embroid inducing culture media under 3 different temperature pretreatments. Better media and pretreatment for embroid production from auther were chosen. The frequency of embroids and regenerated shoots from the dihaploid authers were increased to 27.5% and 8.8%, respectively. The result of ploidy inspecting showed that most of the regenetated shoots were homozygous dihaploids, which suggested that the most embroids were from meiosis gamete and their somatic chromosomes were doubled during the development of the embroids.