惠阳胡须鸡IFN-α、IFN-β基因在大肠杆菌中的表达及其抗病毒活性比较

Expression of IFN-α and IFN-β of HuiYang Chicken in E.coli and antiviral activities analysis

构建了惠阳胡须鸡IFN-α/pGEX-6P-1I、FN-β/pGEX-6P-1的重组质粒,并在BL21中表达,对重组蛋白进行了抗病毒活性测定,结果表明IFN-αI、FN-β重组蛋白的抗病毒活性分别为7.9×105U/mg和6.4×104U/mg,IFN-α的抗病毒活性是IFN-β的12倍。IFN-αI、FN-β与干扰素受体具有不同的结合位点和结合途径可能是使IFN-α和IFN-β的生物学活性存在较大差异的原因。

IFN-α,IFN-β of Huiyang chicken were subcloned into pGEX-6P-1, the recombinant plasmids were transformed into BL21 and expressed. Recombinant IFN-α,IFN-β expressed in E.coli exert high antiviral activities as 7.9×10^5U/mg and 6.4×10^4 U/mg respectively.Antiviral activity of IFN-α is 12 times of IFN-β.The different binding sites to the receptors between IFN-β and IFN-α may induce the different signal transmission and different biological activities.