铜绿微囊藻对5种抗生素敏感性的研究

Study on the Sensitivity of Microcystis aeruginosa to Five Antibiotics

研究了铜绿微囊藻对链霉素、氯霉素、氨苄青霉素、G418和卡那霉素5种常用抗生素的敏感性.结果表明,铜绿微囊藻对这5种抗生素都较为敏感,其中尤以链霉素和氨苄青霉素的抑制效果最好,它们完全抑制铜绿微囊藻生长的浓度都不超过1.0μg/mL,而氯霉素、G418和卡那霉素对藻细胞的致死浓度也不超过10μg/mL.这说明5种抗生素均可作为铜绿微囊藻遗传转化的筛选压力.

The Microcystis aeruginosa was tested for sensitivity to 5 antibiotics., kananycin （Km）. geneticin （G418）, ampicillin （Amp）, chloramphenicol （Cm）,streptomycin （Str）, all of which are frequently applied as selectable markers in plant gene engineering. Growth tests, carried out at different concentrations of the agents above, the result showed that Microcystis aeruginosa were relatively sensitive to Amp, Cm, Km, G418 and Str. the lethal concentration of Cm, Km and G418 were less than 10 μg/mL. Further more,Microcystis aeruginosa is highly sensitive to Amp and Str.0.5 μg/mL Amp or 1.0 μg/mL Str was the lethal concentration of Microcystis aeruginosa in liquid culture. It suggests that Amp.Cm.Str.Km and G418 can be suitable to select regent for Microcystis aeruginosa genetic engineering.