摘要
目的观察不同浓度肿瘤坏死因子攻击下肺泡上皮细胞(A549细胞)存活率和分泌IL-8水平变化。方法A549细胞分为对照组和实验组,分别采用不同浓度TNF-α攻击,培养24h后测定各组细胞存活率、上清IL-8水平。结果不同浓度TNF-α攻击均能明显抑制A549细胞生长,其中5000u·ml^-1浓度组细胞存活率降为(90.1±1.1)%。TNF-α攻击浓度由100u·ml^-1升至1000u·ml^-1时,A549细胞分泌IL-8量由9.1±3.5pg·ml^-1升至203.7±12.4pg·ml^-1,TNF-α攻击浓度超过5000u·ml^-1后IL-8分泌量开始下降。结论以100u·ml^-1~1000u·ml^-1之间浓度的TNF-α进行攻击,A549细胞IL-8分泌量与TNF-α浓度存在剂量一效应关系,反映了急性肺损伤/急性呼吸窘迫综合征状态下肺泡上皮细胞分泌炎性介质情况。
Objective To investigate the effect of tumor necrosis factor-alpha on the secretion of interleukin-8(IL-8) from alveolar epithelial cells(AS49) in vitro. Methods A549 cells were divided into five groups: one control group and four TNF-α groups. After 24 hours' culture,the livability of A549 cells and the secretion of IL-8 were measured by ELISA. Resuits The livability of TNF-αgroups were lower than that of the control group (5000 u· ml^- 1-TNF-α group) (90.1 ± 1. 1) % vs control group (97.9 ± 0.9) %. TNF-α induced a rapidly growing of interleukin-8 in the TNF-α group ( 1000u· ml^ - 1-TNF-α group 203.7 ± 12.4 pg· ml^- 1 vs control group 9.1 ± 3.5 pg· ml^- 1 ). Conclusion When the concentration of TNF-α was 100 u· ml^-1- 1000 u· ml^-1, there was a dose-dependent fashion between the secretion IL-8 and the concentration of TNF-α, which reflects the state of alveolar type Ⅱ epithelial cell in acute luing injury/acute respiratory disease syndrome.
出处
《新乡医学院学报》
CAS
2006年第1期7-8,共2页
Journal of Xinxiang Medical University
基金
国家"十五"重大攻关课题基金资助(NO:2003BA712A07-01)
关键词
肿瘤坏死因子
肺泡上皮细胞
IL-8
tumor necrosis factor-alpha
alveolar epithelial cells
interleukin-8