宁夏枸杞谷胱甘肽过氧化物酶的测定

Determination of glutathione peroxidase in Lycium barbarum form Ningxia

目的研究宁夏枸杞谷胱甘肽过氧化物酶(GSH-Px)的测定方法,以寻求测定枸杞中GSH-Px的最佳条件。方法以0.2mol·L-1的磷酸缓冲液作为提取介质,1.67%的偏磷酸作为沉淀液,用分光光度计分别在410nm、412nm、414nm、416nm、418nm、420nm、422nm波长下比色测定宁夏枸杞中GSH-Px活性。结果宁夏枸杞中GSH-Px在含1mmol·L-1乙二胺四乙酸(EDTA)和1%聚乙烯吡咯烷酮(PVP)的0.2mol·L-1磷酸缓冲液(pH6.24)作为提取介质,反应2min、412nm波长条件下比色测定,活性最高。结论本方法可以准确地测定枸杞中GSH-Px的活性,且重复性好。

Objective To find the optimum conditions of determine glutathione peroxidase （GSH-Pil） in Lycium barbarum. Methods With 0.2 mol· L^-1 PBS used as distilled solution, and 1.67 % metaphosphoric acid used as precipitator, the activity of GSH-Px was determined using colorimetric analysis at wavelength of 410 nm,412 nm,414 nm,416 nm,418 nm, 420 nm, 422 nm respectively with spectrophotometer. Results Extractove , edoi, pf 0.2 mol· 1^-1 PBS（pH6.24） containing 1 retool· 1^-1EDTA-2Na and 1% polyvinglpyoolidone were the suitable condition for the extraction of GSH-Px in Lycium barbarum. The activity of GSH-Px was highest at wavelength of 412 nm after GSH had reacted with DTNB for three mornutes. Conclusion By this method, the activity of GSH-Px can be determined acctLrately and the results keep steady in every test.