期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

大肠癌组织中环氧化酶2基因表达的实时定量PCR检测 被引量：1

Quantification of cyclooxygenase-2 gene expression in colorectal cancer by real-time polymerase chain(reaction)
下载PDF
导出
摘要 目的探讨环氧化酶2(COX-2)在大肠癌发生和发展中的意义。方法建立实时定量RT-PCR方法,采用LightCycler PCR仪检测了28例大肠癌COX-2 mRNA及内参GAPDH mRNA的表达,以COX-2N=(COX-2拷贝数/GAPDH拷贝数)×103表示COX-2 mRNA表达水平。结果肿瘤组织COX-2 mRNA与正常组织表达水平有极明显差异(P<0.05),转移组织较正常组织COX-2mRNA表达有明显差异(P<0.01)。结论COX-2参与了大肠癌的发生和发展,并起到了重要作用。 Objective To investigate the significance of eyelooxygenase-2 （COX-2） in the occurrence and development of colorectal tumor. Methods Real-time quantitative reverse transcription polymerase chain reaction （RQ-RT-PCR） technique was established for detecting COX-2 and GAPDH mRNA expression in tissues from twenty-eight coloreetal cancer patients. Normalized COX-2 expression level （COX 2N） -- （COX-2/GAPDH）× 10^3. Results The expression level of COX-2 mRNA of colorectal cancer was higher than that of normal tissue（P〈0.05）. The expression level of metastatic tissue was higher than that of normal tissue（P〈0. 01）. Conclusion COX-2 plays a significant role in the development of colorectal cancer.
作者 李忠 王海涛 王荣朝 罗光华 朱江 郑璐 董选
机构地区 苏州大学附属第三医院胃肠外科 苏州大学附属第三医院综合实验室 现在新疆维吾尔自治区伊犁哈萨克自治洲奎屯人民医院普外科
出处 《江苏医药》 CAS CSCD 北大核心 2006年第1期17-19,共3页 Jiangsu Medical Journal
关键词 环氧化酶-2 基因 大肠肿瘤 Cyclooxygenase-2 Gene Colorectal tumor
分类号 R512.62 [医药卫生—内科学] R735.34 [医药卫生—肿瘤]
  • 相关文献

参考文献9

  • 1Fukutake M, Nakatsugi S, Zsoi T, et al. Suppressive effects of nimislide, a selective inhibitor of cyclooxygenase-2, on azoxymethane-induced colon carcinogenesis in mice. Carcinogenesis,1998, 1% 1939 1942.
  • 2Tsunozaki H, Yoshinaga K, Kumagai J, et al. Cyclooxygenas-2 overexpression in colorectal cancer is associated with nonpolypoid growth. Jpn J Clin oncol,2002,32:167-171.
  • 3Kawamorl T, Rao CV, Seibert K, et al. Chemopreventive aclivity of celecoxib, a specific cyclooxygenase-2 inhibitor, against colon carinogenesis, Cancer Res, 1998, 58:409- 412.
  • 4Reddy BS, Hirosc Y, I.ubet R, et al. Chemoprevention of coloncancer by specific cycoloxygenase-2 inhibitor, celecoxib, administered during different stages of carcinogenesis. Cancer Res,2000, 60: 29:3-297.
  • 5Sheng H,Shao J ,Washington MK,eI al. Prostaglandin E increases growth and motility of colorectal carcinoma cells. Biol Chore,2001,276 : 18705-18081.
  • 6Cao Y, Pearman AT, Zimmerman GA, et al. Intracellular unesterified arachidonic acid signals apoptosis. Proc Natl Acad ,Sci USA, 2000. 57-64.
  • 7Hao'XP, Bishop AE, Wallace M, et al. Early expresaion of cyclooxygenase-2 during sporadic colorectal carcinogenesis. JPathol, 1999,187 ; 289-301.
  • 8Sheehan KM, Sheahan K, O'Donoghue DP,et al. The relationship between cyclooxygenese-2 expression snd colorectal cancer.JAMA, 1999,28g : 1254-1257.
  • 9于莲珍,郜恒骏,白剑峰,孙谷,赵翰林,孙亮,缪琨,赵志泉.胃黏膜病变组织COX-2蛋白表达的研究[J].江苏医药,2002,28(7):481-483. 被引量：4

二级参考文献6

  • 1Sung JJ,Leung WK,Go MY,et al.Cyclooxygenase-2 expression in Helicobacter pyloriassociated premalignant and malignant gastric lesions[].American Journal of Pathology.2000
  • 2Ratnasinghe D,Tangrea JA Roth MJ,et al.Expression of cyclooxygenase-2 in human squamous cell carcinoma of the esophagus;an immunohistochemical survey[].Anticancer Research.1999
  • 3Yamamoto H,Itoh F,Fukushima H,et al.Overexpression of cyclooxygenase-2 protein is less frequent in gastric cancers with microsatellite instability[].International Journal of Cancer.1999
  • 4Ratnasinghe D,Tangrea JA,Roth MJ,et al.Expression of cyclooxygenase-2 in human adenocarcinomas of the gastric cardia and corpus[].Oncology Reports.1999
  • 5Murata H,Kawano S,Tsuji S,et al.Cyclooxygenase-2 overexpression enhances lymphatic invasion and metastasis in human gastric carcinoma[].The American journal of Gastroenterology.1999
  • 6Hao X,Bishop AE,Wallace M,et al.Early expression of cyclooxygenase-2 during sporadic colorectal carcinogenesis[].Journal of Paleopathology.1999

共引文献3

同被引文献7

  • 1Fukutake M, Nakatsugi S, Zsoi T, et al. Suppressive effects of nimislide, a selective inhibitor of inhibitor of cyclooxygenase-2, on azoxymethane-induced colon carcinogenesis in mice[J]. Carcinogenesis, 1998, 19 (11):1939- 1942.
  • 2Cianchi F,Cortesini C, Bechi P, et al. Up-regulation of eyclooxygenase-2 gene expression correlates with tumor angiogenesis in human colorectal cancer[J]. Gastroenterology, 2001, 121(6):1339-1342.
  • 3Daniel TO, Liu H, Morrow JD, et al. Thromboxane A2 is a mediator of cyclooxygenase-2-dependent endothelial migration and angiogenesis[J]. Cancer Res, 1999, 59(18): 4574-4578.
  • 4Tsunozaki H, Yoshinaga K, Kumagai J, et al. Cyclooxygenase-2 overexpression in colorectal cancer is associated with non-polypoid growth[J]. Jpn J Clin Oncol, 2002, 32 (5): 167-171.
  • 5Hao XP, Bishop AE, Wallace M, et al. Early expression of cyclooxygenase-2 during sporadic colorectal carcinogenesis[J]. J Pathol, 1999, 187(3):295-298.
  • 6Maekawa M,Sugano K, Sano H, et al. Increased expression of cyclooxygenase-2 to -1 in human colorectal cancer and adenomas, but not in hyperplasic polyps [J]. Jpn J Clin Oncol, 1998, 28(7):421-424.
  • 7高随宽,刘占兵,赵建勋,万远廉,刘玉村,徐文怀.人结直肠肿瘤中环氧化酶-2的表达[J].中华普通外科杂志,2000,15(11):663-664. 被引量：3

引证文献1

江苏医药
2006年 第1期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部