人参和氯沙坦对缺血再灌注心肌细胞Bcl-2表达的影响(英文)

Effects of losartan and ginseng on cardiomyocyte Bcl-2 gene expression induced by ischemia and reperfusion

背景心肌细胞凋亡与缺血再灌注损伤直接相关,Bcl-2表达与细胞凋亡密切相关。临床和实验证实人参和氯沙坦均能改善心肌缺血,对缺血再灌注损伤有明显保护作用,但两者对缺血再灌注心肌细胞损伤有何异同?目的探讨人参和氯沙坦对大鼠缺血再灌注心肌细胞内Bcl-2m RNA和蛋白质表达的影响。设计随机对照实验。单位华中科技大学同济医学院附属同济医院心血管内科,华中科技大学同济医学院神经生物学系。材料实验于2002-11/2003-04在华中科技大学同济医学院附属同济医院心血管内科实验室完成。采用健康成年W istar大鼠40只,体质量200～250g,雌雄不限,随机分为假手术对照组,缺血再灌注组,人参治疗组和氯沙坦治疗组,每组10只。方法缺血再灌注组,人参治疗组和氯沙坦治疗组均造模,假手术对照组不造模。氯沙坦治疗组术前2h,术后立即和术后24h分别给予氯沙坦20m g/kg(1m L),灌胃各1次;人参治疗组术前2h,术后立即和术后24h分别给予红参煎剂(1g/m L,1m L/次)灌胃各1次。假手术对照组和缺血再灌注组分别于相同时间给予相同容积的生理盐水灌胃。用原位杂交和免疫组化分别检测人参和氯沙坦治疗后的大鼠缺血再灌注心肌细胞内Bcl-2m RNA和蛋白质含量,并与对照组比较。主要观察指标人参和氯沙坦治疗后的大鼠缺血再灌注心肌细胞内Bcl-2m RNA和蛋白质的表达水平,并与对照组比较。结果实验大鼠40只均进入结果分析。①氯沙坦组与对照组心肌细胞内Bcl-2mRNA,蛋白含量无明显差别(P>0.05)。②人参治疗组Bcl-2m RNA含量和Bcl-2蛋白表达高于缺血再灌注组(P<0.05或0.01)。结论人参治疗组Bcl-2m RNA及Bcl-2蛋白表达均较氯沙坦治疗组高,提示人参防治心肌缺血再灌注损伤抑制心肌细胞凋亡与氯沙坦不同。

BACKGROUND： The studies found that cardiomyocyte apoptosis is related to ischemia-reperfusion injury directly. The clinical and experimental studies proved that ginseng and losartan could improve myocardial ischemia and prevent the ischemia-reperfusion injury. But the comparative study of their effects on cardiomyocyte injury induced by isnhemia and reperfusion has not been reported. OBJECTIVE： To compare the effects of ginseng and losartan on cardiomyocyte Bcl-2 gene expression after ischemia and reperfusion in vivo. DESIGN： Randomized controlled experiment . SETTING： Department of Cardiovascular Internal Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology. MATERIALS： The experiment was carried out in the Laboratory of Cardiovascular Internal Medicine of Tongji Hospital of Tongji Medical College Affiliated to Huashong University of Science and Technology from Novembet 2002 to April 2003. Totally 40 healthy adult Wistar rats＇, weighting 200-250 g, of either gender, were selected and divided into 4 groups： sham operated group, ischemia-reperfusion group, ginseng treated group and losartan treated group＇with 10 rats in each group. METHODS： Rats were modeled in ischemia-reperfusion group, ginseng treated group and losartan treated group but not in sham operation group. 20 mg/kg （1 mL in volume） losartan was given by stomach. The first administration was 2 hours prior to operation. Subsequently, the second and third administrations were given in immediate and 24 hours after operation, respectively; 1 mL of radix ginseng rubra （1 g/mL） was given by stomach. The first administration was 2 hours before operation. Subsequendy, the second and third administrations were given in just and 24 hours after operation, respectively. The rats in ischemia-reperfusion and sham-operated group were given the normal saline with the same volume and at the same time. Immunnhistochemistry and in situ hybridization histochemistry were used to measure mRNA and protein of Bcl-2 gene expression that were compared with those in the control group. MAIN OUTCOME MEASURES： The expression level of Bcl-2 mRNA and Bcl-2 protein in ginseng treated group and losartan treated group. RESULTS： Data of totally 40 rats was entered the final analysis. （1） Content of Bcl-2 mRNA and protein were not significantly different both in ginseng group and control group （P 〉 0.05）. （2） Contents of Bcl-2 mRNA and Bcl-2 protein were-higher in ginseng treated group than those in the ischemia-reperfusion group （P 〈 0.05 or 0.01）. CONCLUSION： Expressions of Bcl-2 mRNA and Bcl-2 protein in ginseng treated group are higher than those in losartan treated group, which suggests that ginseng has not same effect with losartan in inhibiting cardiomyocyte apeptosis and in preventing and curing cardiomyocyte injury induced by ischemia-reperfusion.