胸痹通胶囊对高脂血症家兔心肌的保护作用(英文)

Protection of xiongbitong capsule on myocardium of rabbits with hyperlipemia

背景胸痹通胶囊可增加冠心病患者心肌缺血区的血液灌流,减小心肌梗死面积,改善心电图缺血性S-T改变。目的观察胸痹通胶囊对家兔动脉壁一氧化氮代谢的影响,探讨该药改善心肌血液灌流、发挥心肌保护作用。设计随机分组设计,对照实验。单位山东省潍坊市中医院内一科材料实验于2003-04/2004-02在潍坊医学院生理教研室完成。选用健康成年家兔30只,雌雄各半。随机将动物分为3组正常对照组,模型组,治疗组,每组3只。方法①高脂血症动物模型制备正常对照组喂饲基础颗粒饲料,模型组喂饲造模饲料(基础颗粒饲料+3%猪油+1%胆固醇粉),治疗组除喂造模饲料外,第6周末开始每天用胸痹通胶囊(组方太子参、云苓、瓜蒌、清夏、橘红、石菖蒲、郁金、炙复花、降香、当归、麦冬、五味子)按1粒/kg的剂量加生理盐水10m L灌胃3周。共干预8周。②一氧化氮合酶和一氧化氮代谢产物试剂盒由军事医学科学院放射医学研究所提供,按说明书操作。计算一氧化氮合酶活力,以每分钟每克组织产生的一氧化氮量(nm ol)表示活力;用分光光度计在545nm处测样品光密度,亚硝酸钠溶液制作标准曲线,以μmol/L表示血清一氧化氮代谢产物NO2-,NO3-的浓度。③计量资料组间差异比较采用t检验。主要观察指标各组家兔血管壁组织一氧化氮合酶活力和血清一氧化氮代谢产物浓度比较。结果家兔30只均进入结果分析。家兔动脉壁组织一氧化氮合酶活力及血清一氧化氮代谢产物浓度模型组和治疗组均明显低于正常对照组(P<0.05～0.01),治疗组明显高于模型组(P<0.05)。结论①高脂血症家兔动脉壁一氧化氮系统受损害,血清一氧化氮代谢产物水平降低。②胸痹通胶囊治疗可改善上述状况,起到抗心肌缺血、发挥心肌保护的作用。

BACKGROUND： Xiongbitong capsule （capsule） increases blood flow in myocardial isobemic area in patients with coronary heart disease, lessens the size of myocardial infarction and improves ischemic S-T alternation in electrocardiogram （ECG）. OBJECTIVE： To observe the effect of the capsule on NO metabolism on arterial wail in rabbits and explore the improvement of such drug on myocardial blood flow and protection on myocardium. DESIGN： Randomized group division and control experiment were designed. SETTING： Department of First Internal Medicine, Hospital of Traditional Chinese Medicine, Weifang, Shandong. MATERIALS： The experiment was performed in Teaching-Besearch Boom of Physiology of Weifang Medical College from April 2003 to February 2004. Totally 30 healthy adult rabbits were employed, of either sex and randomized into 3 groups, named normal control, model group and treat- ment group, 3 rabbits in each one. METHODS： （1） Preparation of hyperlipemia （HP） model： In normal control, common granular forage was used. In model group, modeling forage was applied （common granular forage ＋ 3% pig oil ＋ 1% cholesterol powder）. In treatment group, besides modeling forage, at the end of the 6^th week, the capsule [composed of taizishen （Radix Pseudstellariae）, yunling （Poria）, gualou （Fructns Trichesanthis）, qingxia （Rhisoma Pinelliae）, juhong （Exocarpium Cirri Grandis）, shichangpu （Rhizoma Acori Graminei）, yujin （Radix Curcumaca）, zhifuhua （Flos Inulac）, jiangxiang （Lignum Dalbergiae Odoriferae）, danggui（Radix ngelicac Sinensis）, maidong （Radix Lirlopis Platyphyllac） and wuweizi （Fructus Schisandrae）] was administrated everyday for gastric perfusion at 1 pill/kg with 10 mL physiological saline for 3 weeks. The intervention was applied totally for 8 weeks. （2） Nitricoxide synthase （NOS） and NO metabolic product kits were provided from Radiation Medical Institute of Military Academy of Medical Sciences. The operation was based on the instruction. NOS activity was calculated and expressed by NO amount （nmol） produced in one-gram tissue per minutes. Spectrophotometer was used to measure the optic density of sample at 545nm. Sodium nitrite solution was used to prepare standard curve. The concentration of serum NO metabolic products, NO2- and NO3- was expressed with μmol/L,（3） T test was applied for difference comparison a- mong measurement data. MAIN OUTCOME MEASURES： Comparison of NOS activity and concentration of serum NO metabolic products in vessel-waLl tissue in each group. RESULTS： Totally 30 rabbits all entered result analysis. For NOS activity and concentration of serum NO metabolic products in arterial-wall tissue, the results in model group and treatment group were lower remarkably than the control （P 〈 0.05-0.01） and the results in treatment group were higher remarkably than model gsoup.（P 〈 0.05）. CONCLUSION： （1） NO system of arterial wall is damaged in HP rabbits and NO metabolic level is decreased in serum. （2） Xiongbitong capsule improves the above situation and brings the resistance of myocardial isobemia and protection of myocardium into play.