控制性超排卵对小鼠着床期子宫内膜整合素α_vβ_1表达的影响

EFFECT OF CONTROLLED OVARIAN HYPERSTIMULATION ASSISTED BY GnRHa ON IMPLANTATION ENDOMENTRIUM THE EXPRESSION OF INTEGRINS IN MOUSE

目的观察GnRHa长周期辅助超排卵对小鼠子宫内膜组织整合素αV、β1表达的影响,探讨提高IVF-ET妊娠率的方法。方法将36只小鼠随机分成3组。对照组:生理盐水(NS)组;单纯促排卵组:HMG加HCG;GnRHa加促排卵组:GnRHa加HMG加HCG。促排卵和排卵后24、48h,采用免疫组织化学技术检测子宫内膜整合素αV、β1的表达。结果子宫内膜整合素αV、β1表达对照组呈现强阳性,单纯促排卵组稍弱于对照组,2组比较无显著性差异(P>0.05);GnRHa加促排卵组弱于前2组,与前两组分别比较有显著性差异(P<0.05)。结论控制性超排卵药物可以影响着床期子宫内膜整合素αV、β1的表达,从而影响子宫内膜容受性,导致临床妊娠率偏低。

Objective To study the effect of GnRHa assisted controlled ovarian hyperstimulation on the expression of integrins αv andβ1 in Mouse and the strategies to increase the rate of pregnancy in IVF - ET. Methods Thirty-six mice were divided into three groups randomly. Control group： Were perform abdomen injection with 0.9 % salt solution （group NS） ;group with HMG ＋ HCG and group with GnRHa ＋ HMG ＋ HCG. Control group were detected 24h,48h after ovulation,while group with HMG ＋ HCG and group with GnRHa ＋ HMG ＋ HCG. 24 and 48 hours after injection of HCG. The expression of integrins αv andβ1 in implantation endometrium of mouse,was examined by immunohistochemistry techniques. Results There were no significant differences in endometrial thicknesses among the three groups. But more stronger expressions of Integrins αv andβ1 were found in control group and group with HMG ＋ HCG than in group with GnRHa ＋ HMG ＋ HCG.However, in group with GnRHa ＋ HMG ＋ HCG they were expressed obviously lower than the former two groups, the differences among them were significant （P 〈 0.05）. Conclusion The treatment of Controlled Ovarian Hyperstimulation （COH） can inhibit the expressions of Integrins αv andβ1 in implantation endometrium of mouse. Using GnRHa in the period of controlled ovarian hyperstimulation may reduced the endometrial receptivity and further the rate of pregnancy. However,it has no effectiveness on the thickness of endometrium.