摘要
目的检测环孢素A(CsA)与他克莫司(FK506)对大鼠血清及脾组织悬液中白细胞介素18(IL-18)及IL-10含量的影响,探讨CsA和FK506抗排斥反应的不同作用机理。方法将60只Wistar大鼠随机分为3组(每组20只):(1)CsA组:以CsA灌胃(30mg·kg-1·d-1);(2)FK506组:以FK506灌胃(0.6mg·kg-1·d-1);(3)对照组:以生理盐水灌胃(3ml·kg-1·d-1)。用全自动生化分析仪检测肝、肾功能,用酶联免疫吸附试验双抗体夹心法测定大鼠外周血及脾组织悬液中IL-18及IL-10的含量。结果FK506组大鼠血清及脾组织悬液中IL-10含量较对照组明显升高,差异有统计学意义(P<0.05),IL-18与对照组比较明显降低,差异有统计学意义(P<0.05);CsA组血清及脾组织悬液中IL-18明显低于对照组(P<0.05),而IL-10与对照组的差异无统计学意义(P>0.05)。结论FK506可使血清及脾组织悬液中IL-10含量升高,这可能是FK506与CsA抗排斥反应机理不同点之一。
Objective To determine the levels of serum and splenic interleukin 18 （IL-18） and IL-10 in rats treated with cyclosporine A （CsA） and tacrolimus （FK506）, and to explore the different immunosuppressive mechanism of these two agents. Methods Sixty rats were randomly divided into three groups： CsA group： receiving CsA （30mg·kg^-1·d^-1）, gastric irrigation; FK506 group： receiving FK506 （0, 6 mg·kg^-1· d^-1 ）, gastric irrigation; control group： receiving normal saline （3 mg·kg^-1·d^-1）, gastric irrigation, The parameters of liver and renal function were determined by auto-biochemistry analyzer. The serum and splenic IL-18 and IL-40 levels were determined by using ELISA. Results Serum and splenic IL-10 levels in FK506 group were significantly higher than in CsA group and control group （P〈0. 05）. IL-18 levels were decreased significantly in FK506 group as compared with control group （P〈0. 05）. In CsA group, serum and spenic IL-18 levels were decreased significantly （P〈0. 05）, while IL-10 had no significant change as compared with control group （P2〉 0. 05）. Conclusion The increased serum and splenic IL-10 may be one of the causes of taerolimus and CsA with different immunosuppression.
出处
《中华器官移植杂志》
CAS
CSCD
北大核心
2005年第11期676-678,共3页
Chinese Journal of Organ Transplantation
基金
全军医学科研"十五"规划重点课题(01Z061)
