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猪CD80 mRNA定量检测方法的建立 被引量：1

Development of Quantitative Detectection of Porcine CD80 mRNA
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摘要 用RT-PCR技术从猪肺泡巨噬细胞中扩增和克隆了猪CD80基因部分片段,经测序鉴定后,用反向PCR技术构建了CD80的缺失竞争cDNA分子。通过竞争PCR方法,建立了CD80标准竞争曲线,并得到其直线回归方程。本方法操作简单,特异性和敏感性高,可用于猪CD80 mRNA水平的定量检测,从而为分析猪的免疫状态提供了一种重要技术手段。 Porcine CD80 was amplified by RT-PCR from porcine alveolar macrophages and cloned. After se queneing, competitive depletion clones for the cDNA molecules was generated by reverse PCR. Then, the linear regression equation was obtained after construction of the standard competitive curves by quantitative competitive PCR assay. The present study provided an important method to analysis porcine immune status for its simplicity and high specificity and sensitivity during detecting porcine CD80 mRNA level.
作者 周双海 郭鑫 杨汉春 陈艳红 查振林
机构地区 中国农业大学动物医学院
出处 《北京农学院学报》 2005年第4期9-11,共3页 Journal of Beijing University of Agriculture
基金 教育部跨世纪人才基金(2003)资助项目
关键词 猪 CD80 竞争PCR porcine CD80 competitive PCR
分类号 S852.4 [农业科学—基础兽医学]
  • 相关文献

参考文献10

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共引文献16

同被引文献11

  • 1金华利,张富春,李轶杰,张爱莲,王宾.竞争PCR定量检测免疫小鼠细胞因子表达水平[J].生物技术,2004,14(5):44-46. 被引量：1
  • 2张国栋,夏涛,陈小冬,杨在清.猪脂联素mRNA竞争性PCR定量方法的建立[J].华中农业大学学报,2005,24(2):189-191. 被引量：1
  • 3叶巍,邵先安,郑秀娟,陈习武,储以微,熊思东.定量检测CCL20的竞争性内参照RT-PCR的建立及其初步应用[J].复旦学报（医学版）,2005,32(3):359-364. 被引量：3
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引证文献1

二级引证文献1

北京农学院学报
2005年 第4期

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