摘要
运用钙离子荧光指示剂Ｆｕｒａ－２／ＡＭ，检测了粉防己碱（Ｔｅｔ）对成年大鼠心室肌细胞电压依赖性钙通道的影响。结果显示：基础状态下心肌细胞内钙离子（［Ｃａ２＋］ｉ）为１６２．６±７．３ｎｍｏｌ·Ｌ－１，５０ｍｍｏｌ·Ｌ－１氯化钾能使［Ｃａ２＋］ｉ增加至４８０．８±９．３ｎｍｏｌ·Ｌ－１（Ｐ＜０．０１），在无细胞外钙条件下，这种增加作用消失，而预先给予Ｔｅｔ和维拉帕米（Ｖｅｒ）则能阻断高钾升高［Ｃａ２＋］ｉ的作用。结果提示：Ｔｅｔ是通过阻断电压依赖性钙通道而发挥作用的。
The effect of tetrandrine on voltage-dependent calcium channel of ventricular myocyte was investigated by fluorescein Fura-2/AM in rats.It was showed that the concentration Of Ventricular myocyte[ Ca2+]i WaS 162. 6±7.3nmol·L-1.50mmol·L 1 KCl could increase signiflcantly[ Ca2+]i(480.6±9.3nmol·L-1 ,P<0.01),Pretreatment of tetrandrine and verapamil could block KCl-induced increase of [ Ca2+] i.Under the condition of extracellular calcium free,the effect of KCl-induced increase [ Ca2+] i disappeared.The results Suggest that like verapamil, tetrandrine plays it' s pharmacological effects via blocking voltage-dependent calcium channel.
出处
《中国药理学通报》
CAS
CSCD
北大核心
1996年第3期226-228,共3页
Chinese Pharmacological Bulletin
基金
国家自然科学基金
