狗股骨植入EH材料、自体和同种骨的对比研究

A Comparative Study Between EH Composite Material and Autogenic or Allogenic Cancellous Bone after Implantation in the Femora of Dog

目的：评价ＥＨ材料作为骨移植的安全性和有效性。方法：实验用３条杂种狗，无菌条件下在两侧股骨干各钻５个直径为４ｍｍ的圆孔，每侧依次植入表面带有或不带有ＨＡ颗粒的ＥＨ材料、自体松质骨和同种松质骨，第３个孔为空白对照。于术后８周取材。分别用荧光显微镜、光镜和偏振光显微镜观察切片，并用图像分析仪测量新骨面积。结果：（１）植入自体和同种松质骨后的新骨面积分别为８１％和６４％，（２）空白钻孔的新骨面积仅占４０％，（３）ＥＨ材料能与骨组织直接结合。结论：ＥＨ材料具有良好的骨传导性，是一种安全、有效的骨移植替代材料。

Aim: The safety and effectiveness of epoxy resin and hydroxyapatite (EH) composite material as a bone graft was evaluated. Methods: Three dogs were used in this study, five holes of 4 mm in diameter were made on both femoral shafts of each animal under aseptic condition. EH composite material with or without hydroxyapatite granules on its surface, autogenic and allogenic cancellous bone were implanted in turn into the holes on each side separately except the third one which was served as a control. The implants were retrieved in eight weeks postoperatively. Undecalcified sections were observed with fluorescent microscope, light microscope and polarizing microscope. The area of new bone was measured with image analzer. Results: (1) The areas of new bone with autograft or allograft were 81% and 64% respectively. (2) The area of new bone in control holes was only 40%. (3) EH composite material could be directly bonded with bone. Conclusion: EH composite material with good osteoconductivity, is a safe, effective alternative for a bone graft.