人乳头瘤病毒感染与P53及肺耐药蛋白表达在非小细胞肺癌中的关系及其临床意义

The relationship among human papillomavirus infection and the expression of protein P53 and LRP in non-small-cell lung cancer

目的:探讨人乳头瘤病毒(humanpapillomavirus,HPV)感染在非小细胞肺癌(non-small-celllungcancer,NSCLC)发生中的病因学意义及其与P53蛋白和肺耐药蛋白(lungresistanceprotein,LRP)表达之间的相关性。方法:应用聚合酶链反应(PCR)、免疫组化方法(IHC)分别检测76例NSCLC组织中HPVDNA及其E6、E7原癌蛋白,P53和LRP蛋白的表达情况。结果:NSCLC中HPVDNA及其E6、E7原癌蛋白的检出率分别为40.8%、43.4%,2种方法检测的符合率为78.9%;P53和LRP的阳性率分别为63.2%、59.2%,且HPV感染阳性组中P53蛋白阳性表达率(75.8%)显著高于阴性组(46.5%)(P<0.05);P53蛋白表达阳性组中LRP阳性表达率(68.8%)显著高于阴性组(42.9%)(P<0.05);而HPV感染阳性组与阴性组间LRP的表达无显著性差异(P>0.05)。HPV感染与高、中分化程度的NSCLC及吸烟有关;结论:HPV感染可能是NSCLC发生的另一重要病因学因素,且HPV感染可能导致P53基因突变,同时基因突变的P53可能促进肺癌耐药性的增加。

Objective： To investigate the relationship among human papiUomavirus（HPV） infection and the expression of protein P53 and lung resistance protein （LRP） in non-small-cell lung cancer （NSCLC）. Methods： PCR and S-P immunohistochemical （IHC）method were used respectively to examine the expressions of HPV DNA,HPV E6,E7 protein, P53 and LRP in the 76 cases with NSCLC. Results： Of 76 cases with NSCLC,the positive percentage of HPV DNA,HPV E6,E7 protein,P53 and LRP were 40.8%,43.4%,63.2%,59.2% respectively. The coincident rates of both PCR and IHC methods were 78.9%. The percentage of positive of P53 protein was evidently higher in the group of HPV infection（75.8%）than in the negative group（46.5%）（P〈0.05）; the percentage of positive of LRP was significantly higher in the group of P53 positive （68.8%）than in the negative group （42.9%）（P〈0.05）. But LRP expressions were not significantly difference between positive and negative HPV infection groups（P〉0.05）. The expression of HPV was significantly correlated with the high and middle degree of carcinoma differentiation of NSCLC and smoking. Conclusions： HPV infection may be another important etiological factors that result in the occurrence of NSCLC. HPV infection may induce the mutation of P53 gene and the mutative P53 may increase the drug resistance of the lung cancer.