^（131）I标记肝癌单克隆抗体HAb18F（ab＇）_2的NBS法研究

A N-bromosuccinimide Oxidant Method for Labeling F (ab')_2 Fragments of McAb HAb18 by Radioiodine-131

本文研究了以溴代琥珀酰亚胺（ＮＢＳ）为氧化剂的（１３１）Ⅰ标记肝癌单克隆抗体ＨＡｂ１８Ｆ（ａｂ’）２的方法。探讨了反应体系中ＮＢＳ的用量，Ｆ（ａｈ’）２的用量及Ｎａ（１３１）Ⅰ活度三个因素对标记率的影响，并从反应机理上予以解释。

A N-bromosuccinimide (NBS) oxidant method for labeling F (ah' )2 fragments ofMcAb HAb18 by radioiodine-131 was described. The effects of NBS concentration, F (ah' )2concentration and Na131I activity on labeling efficiency were studied.