氯胺酮对家兔TNFα、IL-8的影响

Ketamine inhibits TNFα and IL-8 elevation and attenuates acute lung injury after LPS injection in rabbits

目的研究氯胺酮对TNFαI、L-8水平和急性肺损伤(ALI)的影响。方法采用两次注射LPS的方法复制家兔ALI模型。动物随机分为四组(6只/组):NS组(阴性对照)、LPS组(阳性对照)、氯胺酮1组(K1组)和氯胺酮2组(K2组)。从第2次LPS注射前即刻开始,分别在0、2、4、6、8 h测定各组家兔血浆TNFαI、L-8浓度及8 h时支气管肺泡灌洗液(BALF)中TNFα、IL-8浓度,同时进行各组肺组织病理学检查。结果NS组各指标无明显变化(P>0.05)。LPS组血浆和BALF中TNFαI、L-8浓度均显著高于NS组(P<0.01)。双肺明显肿胀,表面有点片状出血,轻挤切面有水肿液溢出,镜下有明显肺不张和肺泡萎陷,肺间质增厚,内有大量白细胞浸润,肺血管淤血。K1、K2组的变化类似LPS组,但程度更轻,K2组又轻于K1组。结论氯胺酮可抑制家兔血浆和BALF中TNFαI、L-8浓度的升高,减轻ALI程度。

Objective To investigate the effects of ketamine on TNFα and IL-8 concentration and on acute lung injury（ALl） after LPS injection in rabbits. Methods The LPS-indueed ALI model was reproduced by two interval venous injection of LPS in rabbits. The rabbits were randomly divided into four groups, i. e, group NS, group LPS,group ketamine 1（group K1） and group ketamine 2 （group K2 ）. The plasma TNFα and IL-8 concentrations were measured before（0h） and 2,4,6,8 hours after second LPS injection. The concentration of TNFα and IL-8 in bronchoalveolar lavage fluid（BALF） was measured and lung pathologic study was taken at the end of the experiment. Results No significant changes were found in group NS（P 〉 0.05）. In group LPS, however, TNFα and IL-8 concentration in both plasma and BALF were significantly elevated compared with group NS （ P 〈 0.01 ）. Lung pathological changes were that of typical inflammation, including edema, dotted or parched surface haemorrhage, spillage fluid from curting surface under after tender squeezing. Microscopic pathological changes consisted of pulmonary atetecrasis, alveolar collapses, increased pulmonary interstitial thickness with the infiltration of numerous leukocytes,and congesrion of mlcrovessels. Compared with group LIPS, the obove changes in group K1 and K2 were similar but attenuated, with the latter being even more improved. Conclusion Ketamine inhibits the elevation of plasma and BALF TNFα and IL-8 concentration,and attenuates acute lung injury after LPS injection.