幽门螺杆菌对人上皮细胞的吸附

Adherence of Helicobacter pylori to Human Epithelicl Cell

幽门螺杆菌对人胃粘膜的吸附是实现其感染的第一步。本研究以人上皮细胞株ＨＥｐ—２为材料对幽门螺杆菌吸附上皮细胞的性能进行了探讨，结果显示，幽门螺杆菌ＹＣ—１１Ａ株具有特异吸附于ＨＥｐ—２细胞表面的性能，其吸附能力在与ＨＥｐ—２细胞共孵育３ｈ后达到高峰，吸附率达８１％，此后吸附率虽无显著提高，但单个ＨＥｐ—２细胞吸附的幽门螺杆菌数量却随孵育时间的延长而有明显增加。在大气环境中与在含５％氧气的微氧环境中幽门螺杆菌对ＨＥｐ—２的吸附无显著差异。抗幽门螺杆菌优势抗原单克隆抗体不能有效阻断幽门螺杆菌对ＨＥｐ—２的吸附。

Adherence properties of Helicobacter pylori to human epithelial cell were studied by using HEp-2cell. The results indicated that H. pylori YC-11A was able to adhere HEp-2 cell specifically and its adherence effciency reached to the highest(81%)in 3h after incubation with HEp-2 cell. There was no significant difference between adherence in air and in 5% oxygen.The monoclonal antibodies specific to H. pylori predominant antigens had no inhibitional activities on adherence of H.pylori to HEp-2 cell.