摘要
本文利用高效液相色谱（ＨＰＬＣ）柱前邻苯二甲酝酿（ＯＰＡ）自动衍生、自动进样程序测定脑组织中谷氨酸、天冬氨酸、甘氨酸、牛磺酸和ｒ－氨基丁酸含量。该法的最低检出限为１０ｎｍｏｌ／ｍｌ，５种氨基酸峰面积测定值的变异系数平均为２．０±１．３％，线性相关系数平均为０．９７±０．０３。
Glutamate, asparate, glycine, taurine and GABA in brain were seperated and determined by HPLC system of the automatic processes of precolumn o phthaldialdehyde derivatization and injection. Average coefficient of variation for peak areas of 5 amino acids was 2.0±1.3% with the detection limit of 50ng. Average line coorelation coefficient was 0.97±0.03.
出处
《河北医科大学学报》
CAS
1996年第3期132-134,共3页
Journal of Hebei Medical University
基金
青年科研基金
关键词
氨基酸
自动衍生
高效液相色谱
脑组织
HPLC
o phthaldialdehyde
amino acids
automatic derivatization
automatic injection
