胰岛素样生长因子1对早孕滋养细胞增殖的作用及机制研究

The effect of insulin-like growth factor 1 on prandoliferation activity of human trophoblast cell in vitro.

目的探讨胰岛素样生长因子1(IGF-1)对体外培养的早孕滋养细胞增殖的影响,以及滋养细胞增殖的细胞内信号转导机制。方法取原代培养传代后生长良好的滋养层细胞,加入不同浓度的IGF-1继续培养,用四唑盐(MTT)比色法测定细胞的增殖活性,并且以ERK(细胞外信号调节蛋白激酶)通路的特异性抑制剂U0126处理细胞,间接反映ERK通路的作用。结果①与对照组相比,IGF-1浓度≥1nM时,其促进滋养细胞增殖效果有显著性意义(P<0.05),且在0.1-100nM范围内,此种作用与浓度呈正相关。②ERK通路阻滞剂U0126可抑制滋养细胞的增殖(P<0.05),并且可显著抑制IGF-1对滋养细胞的促增殖作用(P<0.05)。结论①IGF-1对滋养层细胞的增殖活性具有促进作用。②ERK通路可能是滋养细胞增殖过程中的主要信号转导通路之一。

Objective： Our aim was to study the effect of insulin - like growth factor 1 and ERK signal pathway on proliferation activity of human trophoblast cell in vitro. Methods ： Growing - well trophoblast cells were selected, cultured with IGF - 1 of different concentration, the proliferation activity were measured by MTT assay. U0126, the specific inhibitor of ERK signal pathway was used to reflect indirectly the possible involvment of ERK signal pathway. Results： There were siginificant difference between experimental groups of insulin- like growth factor 1 above lnM and the control one, and the effect has positive relation with the concentrations at range of 0. 1 - 100nM. U0126, the specific inhibitor of ERK signal pathway not only inhibited the proliferation activity of trophoblast cell but also restrained significantly the effect of IGF - 1 on trophoblast cell. Conclusion： Insulin - like growth factor 1 has promoting effect on the growth of trophoblast cell. ERK/MAPK pathway is maybe one of important signaling pathways in the proliferation activity on trophoblast cells of human early pregnancy in vitro.