补救ICSI的妊娠结局分析(附补救后冷冻胚胎复苏妊娠一例)

The analyse result of later ICSI (one case was pregnancy after thawing later ICSI embryo transfer).

目的对体外受精(IVF)完全失败的周期及时补行卵细胞浆内单精子显微注射(rescue ICSI)后的临床妊娠结局的探讨和分析。方法IVF受精失败后及时将MⅡ卵母细胞进行补救ICSI。结果补行ICSI后10周期中移植9个周期19个胚胎,妊娠1例双胎,种植为10.5%;有2个周期有冷冻胚胎并行复苏胚胎移植,单胎妊娠1例。补救ICSI累计移植胚胎25个,种植3个累计种植率为12.0%,但2例均以流产告终。结论补救ICSI妊娠率低、流产率高与卵子过度老化有关,冷冻复苏补救ICSI的胚胎,可以消除胚胎发育与子宫内膜发育不同步的因素。在IVF受精率低的周期中可以将未受精的卵母细胞补行ICSI后行胚胎冷冻-复苏移植,可以增加取卵周期的妊娠率。

Objective： To discuss and analyse the outcome of pregnancy by rescue intracytoplasimc sperm injection （ICSI） after complete fertilization failure during in vitro fertilization （IVF） cycles, Methods ： After fertilization failure during IVF cycles, M Ⅱ oo- cytes were re -fertilitzed by ICSI, Results： Totle rescue ICSI were 10 cycles, 19 embryos were transferred to the patient during 9 cycles. Implantation rate were 10, 5% （2/19）. 6 embryos were transferred to the patient during 2 frozen -thawed embryos transfer （FET） cycles, and 1 was clinical pregnancy, All of the transferred embryos of rescue ICSI were 25, and the implantation rate were 12. 0% （3/25）, both of the 2 pregnancies in the rescue ICSI had abortion, Conclusion： The pregnancy rate and implanting rate of rescue ICSI is poor and abortion rate is high because of exorbitant maturity of oocytes. Transferred frozen - thawed embryos during rescue ICSI maybe dispel the badness factor for growth of embryo and endometrium were not synchronization, During the IVF cycles with the poor fertilized rate. unfertilized oocytes were re - fertilizaton then thansferred frozen - thawed embryos, it may be increase pregnancy rate of retrieval oocyets cycles,