微生物来源抗肿瘤活性化合物SIPI-100-2的研究

Studies on the Anti-tumor Agent SIPI-100-2 from a Microorganism

目的:分离链霉菌SIPI100菌株生物合成的具有抗肿瘤活性的化合物。方法:优化发酵条件,进行大规模发酵;发酵液经离心、乙酸乙酯萃取、硅胶柱层析等处理,从发酵液中分离出化合物SIPI1002,并测定化合物的图谱数据和生物学活性。结果:通过理化性质、质谱、紫外、红外和核磁共振等图谱数据的分析,确定化合物SIPI1002的化学结构;生物学研究发现其具有中等强度的抗肿瘤活性。结论:链霉菌SIPI100产生的活性化合物SIPI1002属于安莎类抗生素,与文献报道的17Odemethylgeldanamycin结构一致。

AIM： To isolate anti-tumor agents from Streptomyces hygroscopicus R-100. METHODS： The fermentation technology of Streptomyces hygroscopicus R-100 was further improved and the broth was obtained in a large volume; using ethyl acetate extraction, silica gel column chromatography, erystallization etc., compound SIPI-100-2 was isolated from its broth; the spectra data of SIPI-100-2 were determined and its biological activity was studied. RESULTS： By the analyses of its physico-chemical properties, MS, UV, IR and NMR spectra data, the structure of SIPI-100-2 was identified; the study on the biological activity of SIPI-100-2 found that it had moderate anti- tumor activity. CONCLUSION： SIPI-100-2 produced by Streptomyces hygroscopicus R-100 is identical with 17-O-demethylgeldanamyein and its new anti-tumor activity is found for the first time.