摘要
采用氮蓝四唑光照法比较了几种虫草无性型及其相关真菌的SOD酶比活力及粗酶含量，同时应用聚丙烯酰胺凝肢电泳法进行了活性显示。结果表明，不同菌种，SOD酶谱差别较大，而分离自冬虫夏草的HS系列菌株和虫草簇孢SIN-1菌株图谱较接近；蝉拟青霉（PC-1）干重的SOD酶比活力最高，达874．3U·g^-1，其次是细脚拟青霉（PT-1）干重的为537．6U·g^-1，HS-1菌株干重的SOD酶比活力为466．9U·g^-1。供试菌株的粗酶含量占干重的百分比在0．73％～2．50％之间。综合酶比活力和含量考虑，蝉拟青霉和HS-1菌株在抗氧化方面具有较好的开发应用前景。
In this paper, the activity and crude conten.t of SOD from. several anamorphic strains of Cordyceps and its allies were compared by NBT-illumination method, and enzyme activity.were showed by polyacrylamide gel electrophoresis. The results showed that SOD zymograms of some species were different, and HS-0- HS=8 strains and SIN-1 all originated from Cordyceps sinenesis had a strong zymogram at same site. PC-1 strain of Paecilomyces cicadae, the traditional Chinese medicine, got the highest enzyme specific activity （874. 3 U·g^-1, dry Weight） among the tested samples,PT-1 strain ofPaecilomyces tenuipes and HS-1 strain were 537.6＇ and 466.9 U·g^-1 ,dry weight,respectively. Crude enzyme contents in dry weight of all strains were 0. 73% -2.50%.
出处
《安徽农业大学学报》
CAS
CSCD
北大核心
2006年第1期90-93,共4页
Journal of Anhui Agricultural University
基金
国家自然科学基金项目(30570004)资助
关键词
虫草
无性型
超氧化物歧化酶
氮蓝四唑光照法
Cordyceps
anamorph
NBT-illumination method
SOD
polyacrylamide gel electrophoresis
