超高压对CB_3V毒力及免疫原性的影响

Effects of high hydrostatic pressure on virulence and antigenicity of Coxsackie virus B_3

目的:通过超高压(HHP)对柯萨奇病毒B3(CB3V)毒力和病毒免疫原性的影响,探讨既能降低病毒毒力又能保留病毒免疫原性的有效方法,为制备CB3V疫苗提供新的途径。方法:病毒毒力检测采用50％组织细胞感染量试验(TCID50)法;病毒免疫原性检测采用巨噬细胞吞噬功能测定、抗体检测、T淋巴细胞转化实验。结果:压力达到680 MPa时,病毒毒力降低,病毒TCID50由10-5上升为10-4;高于700 MPa时,病毒被灭活。对病毒进行加热、压力减毒及压力灭活,小鼠巨噬细胞吞噬功能、抗体产生量及T淋巴细胞转化率与盐水对照组比较差异有显著性(P<0．05),加热组、减毒组与灭活组之间比较差异无显著性(P>0．05)。结论:CB3V对压力的抵抗力强,灭活CB3V的临界压力为700 MPa;压力灭活的CB3V免疫原性不受影响。

Objective To evaluate the influences of high hydrostatic pressure （HHP） on the virulence and antigenicity of Coxsackie virus Ba （CBaV） , to explore a new physiological method to develop an active vaccine against Coxsackie virus, Methods The phagocytosis of the macrophages in mice to the CBa V and the TCIDs0 of the CBaV before and after treated by HHP were examined. Results TCID50 increased from 10^-5 to 10^-4 when treated by 680 MPa; viruses were inactivated at 700 MPa. Viruses were also inactivated by the pressure less than 700 MPa, but needed prolonged time. The phagocytic function, antibody and T lymphocyte transformation rate in experimental mice （heated, attenuated, and inactive viruses） exhibited a significant difference compared with control mice （P（ 0.05）. Conclusion CB3V has a strong resistance to HHP. The inactivated pressure is about 700 MPa, however, the antigenicity of the virus is not influenced by the same value of HHP.