碟脉灵注射液对大鼠心肌缺血再灌注损伤的保护作用

Protective effects of Diemailing Injection on myocardial ischemia-reperfusion injury in rats

目的:观察碟脉灵注射液(DMLI)对大鼠心肌缺血再灌注损伤的保护作用。方法:在体大鼠结扎冠状动脉前降支30 min,再灌注24 h造成心肌缺血再灌注模型,计算心肌梗死范围(MIS),测定血清天冬氨酸氨基转移酶(AST)、乳酸脱氢酶(LDH)、心肌型肌酸激酶同功酶(CK-MB)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性及丙二醛(MDA)含量,并测定血浆前列环素(PGI2)及血栓素A2(TXA2) 水平。结果:与再灌模型组比较,DMLI 5、10 mL·kg-1组MIS明显缩小,血清AST、LDH、CK—MB活性及 MDA含量明显降低,SOD及GSH—Px活性明显升高,血浆TXA2水平明显下降,PGI2水平及PGIz／TXA2比值明显增高。结论:DMLI对大鼠心肌缺血再灌注损伤具有明显保护作用,可能与其增强抗氧化酶活性,减少自由基对心肌的氧化损伤,纠正PGI2／TXA2失衡等机制有关。

Objective To observe the protective effects of Diemailing Injection （DMLI） on myocardial ischemiareperfusion injury in rats. Methods The myocardial ischemia-reperfusion model was induced by 30 min left anterior descending coronary occulusion and 24 h reperfusion in open-chest anesthetized rats. The changes of aspartate aminotransferase （AST）, lactate dehydrogenase （LDH）, MB isoenzyme of creatine kinase （CK-MB）, superoxide dismutase （SOD） and glutathione peroxidase （GSH-Px） activities and malondialdehyde （MDA） contents in serum, and prostaeyeline （PGI2） and thromboxane A2（TXA2） levels in plasma were determined. Results In rats treated by DMLI （with dose of 2.5, 5 and 10 mL · kg^-1 i.v. at 30 min after coronary occulusion）, the myocardial ischemia size （MIS） was significantly reduced, the AST, LDH and CK-MB activities in serum and the TXA2 level in plasma were declined, while PGI2 level in plasma and PGI2/TXA2 ratio were increased significantly. In addition, the LPO content in serum declined, SOD and GSH-Px activities in serum were increased markedly. Conclusion DMLI has protective effects on myocardial ischemia-reperfusion injury through improving free radicals metabolism, decreasing TXA2 level in plasma, increasing PGI2 level in plasma and PGI2/TXA2 ratio.