基质金属蛋白酶-2及其组织抑制剂和微血管密度在子宫内膜癌中的表达

Expressions of matrix metalloproteinases-2 and its tissue inhibitor-2 and microvessel density in endometrial carcinoma

目的,通过研究基质金属蛋白酶-2(MMP-2)及其抑制剂(TIMP-2)和微血管密度(MCV)在子宫内膜癌组织中的表达,探讨其与间质微血管密度及与子宫内膜癌浸润、转移的关系。方法:采用SP法检测 8例正常子宫内膜和33例子宫内膜癌患者MMP-2、TIMP-2及MVD。结果:MMP-2、TIMP-2及MVD在正常子宫内膜及子宫内膜癌组织中均有表达,且在子宫内膜癌中的表达明显高于正常子宫内膜(P<0．05),G1、 G2、G3不同病理分级表达差异无显著性(P>0．05);MMP-2表达随手术病理分期的升高而增强(P<0．05); TIMP-2在子宫内膜癌中的表达随着肌层浸润程度的增加而表达增强;MVD的表达随着肌层浸润程度的增加表达明显增强(P<0．05)。淋巴结有转移者和无转移者各因子的表达差异无显著性。结论:MMP-2／TIMP-2之间的动态平衡失调促进了子宫内膜癌的侵袭和转移;肿瘤微血管形成的强度与肿瘤侵袭能力有关,提示血管生成是子宫内膜癌发生浸润、转移的重要条件。

Objective To study the expressions of matrix metalloproteinases-2 （MMP-2） and its tissue inhibitor-2 （TIMP-2） and microvessel density （MVD） in human endometrial carcinoma tissues and their relations with the invasion and metastasis of endometrial carcinoma. Methods lmmunohlstochemistry was used to measure the MMP-2, TIMP-2, and MVD protein levels in 33 patients with endometrial carcinoma and 8 normal endometrial samples. Results The MMP-2, TIMP-2 and MVD protein expressed in both normal endometrial samples and endometrial carcinoma. The positive expression proportions of MMP-2, TIMP-2, and MVD protein in endometrial carcinoma tissues were higher than those in normal endometrial samples （P〈0. 05）, but they did not show different expression with the histologic grade （G1, G2 and G3）. Semi-quantitative analysis revealed MMP-2 staining scores in tumor cells were significantly associated with the presence of surgical pathologic staging （P〈0. 05）, while TIMP-2 staining scores in tumor cells were significantly associated with the depth of myometrial invasion （P〈0. 05）. MVD staining scores in tumor cells were also significantly associated with the depth of myometrial invasion （P〈0.05） . But these factors had no significant association with the presence of lymph node metastasis. Conclusion The expression maladjustment between MMP-2 and TIMP-2 may accelerate the tumor invasion and metastasis; the increasing expression of MVD in tumor cells may be associated with the depth of myometrial invasion, which suggests that angiogenesis plays an important role in invasion and metastasis of endometrial carcinoma.