摘要
对侵染广东省阳西县田间辣椒的黄瓜花叶病毒(cucumber mosaic wirus,CMV)的CP及2b基因进行序列分析,结果表明其CP基因与CMV亚组Ⅰ、亚组Ⅱ各株系之间的核苷酸同源率分别为90.9%~93.8%和76.1%~76.9%。氨基酸同源率分别为92.7%~97.7%和72.4%~78.1%;其2b基因与CMV亚组Ⅰ、亚组Ⅱ之间的核苷酸同源率分别为83.9%~90.2%和64.0%~67.7%,氨基酸同源率分别为79.3%~85.7%和50.5%~53.5%,由此确认该CMV分离物属于亚组Ⅰ成员.此外,CMV亚组Ⅰ成员的CP及2b基因中分别具有一段长为102bp及54bp的高度保守序列,这为转基因诱导寄主转录后基因沉默(post-transcriptional gene silencing,PTGS)CMV育种研究提供了参考.
The cucumber mosaic virus (CMV) infecting pepper in Yangxi county, Guangdong Province (CMV-gd) was confirmed as a subgroup-I member by sequencing its coat protein (CP) gene and 2b genc. The results showed that CMV-gd shared CP gene nucleotide identities of 90.9%-93.8% and 76.1% -76.9% ( deduced amino acid identities of 92.7% -97.7% and 72.4% -78.1% ) compared with some CMV strains of subgroup I and II respectively, while 2b gene nucleotide identities of 83.9% -90. 2% and 64.0% -67.7% ( deduced amino acid identities of 79.3% -85.7% and 50. 5%-53.5% ) respectively. Furthermore, a 102 bp highly conserved sequence in CP gene and 54bp in 2b gene of CMV subgroup I member were found. It was suggested that they would provide useful information for the anti-CMV strategy via transgene-induced plant post-transcriptional gene silencing(PTGS).
出处
《华南农业大学学报》
CAS
CSCD
北大核心
2006年第1期51-54,共4页
Journal of South China Agricultural University
基金
广东省自然科学基金(E04025)