侵染广东辣椒的黄瓜花叶病毒的CP及2b基因序列分析

Sequencing of CP and 2b Gene of Cucumber Mosaic Virus Infecting Pepper in Guangdong Province

对侵染广东省阳西县田间辣椒的黄瓜花叶病毒（cucumber mosaic wirus，CMV）的CP及2b基因进行序列分析，结果表明其CP基因与CMV亚组Ⅰ、亚组Ⅱ各株系之间的核苷酸同源率分别为90.9％～93.8％和76.1％～76．9％。氨基酸同源率分别为92．7％～97．7％和72．4％～78．1％；其2b基因与CMV亚组Ⅰ、亚组Ⅱ之间的核苷酸同源率分别为83．9％～90．2％和64．0％～67．7％，氨基酸同源率分别为79．3％～85．7％和50.5％～53.5％,由此确认该CMV分离物属于亚组Ⅰ成员．此外，CMV亚组Ⅰ成员的CP及2b基因中分别具有一段长为102bp及54bp的高度保守序列，这为转基因诱导寄主转录后基因沉默（post-transcriptional gene silencing，PTGS）CMV育种研究提供了参考．

The cucumber mosaic virus （CMV） infecting pepper in Yangxi county, Guangdong Province （CMV-gd） was confirmed as a subgroup-I member by sequencing its coat protein （CP） gene and 2b genc. The results showed that CMV-gd shared CP gene nucleotide identities of 90.9%-93.8% and 76.1% -76.9% （ deduced amino acid identities of 92.7% -97.7% and 72.4% -78.1% ） compared with some CMV strains of subgroup I and II respectively, while 2b gene nucleotide identities of 83.9% -90. 2% and 64.0% -67.7% （ deduced amino acid identities of 79.3% -85.7% and 50. 5%-53.5% ） respectively. Furthermore, a 102 bp highly conserved sequence in CP gene and 54bp in 2b gene of CMV subgroup I member were found. It was suggested that they would provide useful information for the anti-CMV strategy via transgene-induced plant post-transcriptional gene silencing（PTGS）.