摘要
研究了在碱性介质和阳离子表面活性剂CTMAB存在下,牛血红蛋白催化鲁米诺-铁氰化钾的化学发光行为和化学发光反应的条件,提出一种流动注射-化学发光法测定牛血红蛋白的新方法.该方法测定牛血红蛋白的线性范围为5.18×10^-10~1.29×10^-6 mol/L,r^2=0.9985;检出限(RSN=3)为1.2×10^-10 mol/L;回收率为(100±10)%;dRSD≤5%(n=6).
The chemiluminescence(CL) characteristics of Hemoglobin(bovine) was studied using flow injection method. The experimental conditions for the Luminol-potassium ferricyanate-hemoglobin-CTMAB CL system were optimized. According to the relations between CL intensity and hemoglobin concentration, a new method was established for the determination of Hemoglobin. The linear range for Hemoglobin was 5.18×10^-10~1.29×10^-6 mol/L, r^2 = 0.998 5; the detection limit was 1.2×10^-10 mol/L(RSN = 3)and the recovery range was between 90 % and 110 % and RSD was lower than 5 %(n=6).
出处
《华中科技大学学报(自然科学版)》
EI
CAS
CSCD
北大核心
2006年第1期114-117,共4页
Journal of Huazhong University of Science and Technology(Natural Science Edition)
基金
国家自然科学基金资助项目(20005005)
关键词
化学发光
流动注射
牛血红蛋白
chemiluminescence
flow-injection
hemoglobin bovine