复合酶法提取稻麸蛋白工艺的优化

Optimization of technology for extracting rice bran protein with different enzymes

运用配料试验设计,采用纤维素酶、复合蛋白酶、植酸酶提取米糠分离蛋白,建立复合酶配合比例与蛋白得率之间的数学模型,确定最佳配比为:纤维素酶65.84%、蛋白酶18.52%、植酸酶15.64%,最佳反应条件为:复合酶温度45℃、底物浓度20%、酶添加量4%、pH值5.5、水解时间2 h,最终蛋白收率可达86%以上。

An experimental method based on controlled mixture ratio of different enzymes in order to improve the yield of rice bran protein separation was proposed. The model between rice bran protein separation and mixture ratio with cellulase, proteases and phytase was established. The optimal proportions of enzymes were cellulase 65.84%, proteases 18.52%, phytase 15.64%. The optimum extracting conditions with rice bran protein yield over 86% were obtained by adding 4% of the enzymes, substrate mass ratio to defatting rice bran by dissolving at 20% in water and incubating 2h at 45℃ and pH 5.5.