子宫颈癌术中髂内动脉灌注化疗的组织病理学变化

Histopathologic change of cervical cancer cells after introoperative internal iliac arterial infusion chemotharepy

目的探讨子宫颈癌术中髂内动脉灌注顺铂对宫颈癌的近期疗效及其作用机制。方法用免疫组织化学法及TdT介导的dUTP缺口末端标记技术,检测25例术中行髂内动脉灌注化疗的子宫颈癌患者(灌注组)肿瘤组织中癌细胞的凋亡及增殖情况,并选择同期手术治疗的宫颈癌患者25例(对照组)作为对照。结果灌注组肿瘤细胞的细胞增殖指数(PI)为(46.34±18.76)%,略低于对照组的(58.48±16.54)%(P>0.05),灌注组中肿瘤细胞的凋亡指数(AI)为(17.67±10.32)%,明显高于对照组的(6.92±5.76)%(P<0.05)。结论子宫颈癌术中通过髂内动脉灌注化疗,可以诱导子宫颈癌细胞发生凋亡,并可能降低其增殖活力。

Objective To study the early effect and mechanism of introoperative internal iliac arterial infusion chemotharepy on cervical cancer. Methods Twenty-five patients with cervical cancer received introoperative internal iliac arterial infusion chemotharepy composed of the infusion group, another 25 patients with cervical cancer who were underwent surgical operation were studied as the control group. Apoptotic cell apoptosis was examined by TUNEL. The expression level of proliferating cell nuclear antigen （PCNA） was detected by immunohistochemical staining. Results Compared with the control group, the apoptosis index （AI） of cancer ceils was higher in the infusion group （17.67% vs 6.92%） （P〈0.05）, and the PCNA index （PI） was lower in the infusion group （46.34% vs 58.48%）, but had no statisitcal significance （P〉0.05）. Conclusions Introoperative internal iliac arterial infusion chemotharepy can induce apotosis of cervical cancer cell and may decrease it＇s proliferative activity.