高度富集黄酮类成分的贯叶连翘提取物抗抑郁作用

Antidepressant Activity of Extract of Hypericum perforatum with Enriched Flavonoids

目的探讨贯叶连翘提取物中高度富集的黄酮类成分的抗抑郁作用。方法采用小鼠强迫游泳实验、小鼠悬尾实验、开野实验和拮抗利血平所致的体温降低实验,分别以小鼠不动时间、自主活动数和体温下降值作为评价指标。结果在强迫游泳和悬尾实验中,40,80,160,240mg/kg剂量组贯叶连翘提取物均能显著缩短小鼠不动时间,20mg/kg剂量组无显著性差异;开野实验结果表明给药后小鼠的自主活动不同程度地减少;在利血平拮抗实验中,30,60,120mg/kg剂量组在3h,4h和5h各时间点体温下降值与模型对照组相比有显著性差异,240和480mg/kg两剂量组在各测定时间点体温下降值与模型对照组相比均有显著性差异。结论高度富集总黄酮的贯叶连翘提取物(弃除贯叶金丝桃素)有抗抑郁作用。

Objective To investigate the antidepressed effects of the extract of Hypericum perforatum with enriched flavonoids. Methods The forced swimming test （FST） , tail suspension test （TST） , open field activity （OFA） test and reserpine - induced hypothermia test were carried out to determine the antidepressant activity in mice. Mice immobility duration in FST and TST , spontaneous activity frequency in OFA , and body temperature decrease in reserpine antagonistic test were observed. Results Extract at the dosages of 40, 80, 160 and 240 mg/kg dosage except the dosage of 20 mg/kg, could significantly shorten mice immobility time in FST and TST. Extract could reduce mice spontaneous activity frequency to some degree in OFA. Compared with the model group, extract at the dosages of 30, 60 and 120 mg/ kg could remarkably inhibit the decrease of body temperature in reserpine antagonistic test at 3^rd, 4^th and 5^th hour, so did the extract at the dosages of 240 and 480 mg/kg. Conclusion Extract of Hypericum perforatum with enriched flavonoids （free of hyperforin） exerts an antidepressant activity in animal models.