大孔吸附树脂分离纯化威灵仙总皂苷的工艺研究

Study on Separation and Purification of Total Saponins from Radix Clematidis with Macroporous Absorptive Resin

目的研究大孔吸附树脂分离纯化威灵仙总皂苷的工艺条件。方法威灵仙用70%乙醇回流提取后,上D101型大孔树脂,水洗后分别用30%,50%,70%乙醇洗脱,以威灵仙总皂苷的洗脱率为指标,考察大孔树脂分离纯化威灵仙总皂苷的吸附性能和洗脱参数。结果威灵仙总皂苷主要富集于50%乙醇洗脱液部分,大孔吸附树脂的吸附容量为53.36mg/g,洗脱率达71.26%。结论采用大孔吸附树脂分离纯化威灵仙总皂苷是可行的,优选洗脱条件为用水洗去水溶性杂质,50%乙醇洗脱总皂苷。

Objective To establish a method for the separation and purification of total saponins from Radix Clematidis by column chromatography of macroporous absorptive resin. Methods Radix clematidis was extracted with 70 % ethanol and then the extraction was purified with D101 macroporous absorptive resin. After eluting with distilled water and 30 % , 50 % and 70 % ethanol, absorption capability and elution parameters of macroporous absorptive resin were measured by the indexes of elution rate and refinement rate of total saponins. Results Total saponins of Radix Clematidis were enriched in the 50 % ethanol eluted solution fraction. The elution rate of macroporous absorptive resin was 71.26 % and absorption capability was 53.36 mg/g. Conclusion It is feasible to use macroporous absorptive resin for the isolation and purification of total saponins from Radix Clematidis. The optimum elution condition is： removing water- soluble impurity by water, eluting total saponins with 50 % ethanol.