摘要
Objective: To study the expression level of TRF1 (telomeric repeat binding factor 1) protein in human acute leukemia and relationship between expression level of TRF1 protein and telomerase. Methods: A quantitative Western-Blot technique was developed using anti-TRF133-277 monoclonal antibody and GST-TRF1 purity protein as a standard to further determine the ex-pression level of TRF1 protein in total proteins extracted from clinical specimens. Results: Bone marrow tissues of 20 acute leukemia patients were studied, 11 healthy donors’ bone marrows were taken as a control. The expression level of TRF1 protein was significantly higher (P<0.01) in normal bone marrow ((2.217±0.462) μg/μl) than that of acute leukemia patients ((0.754±0.343) μg/μl). But there was no remarkable difference between ALL and ANLL patients ((0.618±0.285) μg/μl vs (0.845±0.359) μg/μl, P>0.05). After chemotherapy, TRF1 expression level of patients with complete remission elevated ((0.772±0.307) μg/μl vs (1.683±0.344) μg/μl, P<0.01), but lower than that of normal ((2.217±0.462) μg/μl, P<0.01). There was no significantly difference after chemotherapy ((0.726±0.411) μg/μl vs (0.895±0.339) μg/μl, P>0.05). TRF1 expression level of patients with complete remission is higher than that of patients without complete remission ((1.683±0.344) μg/μl vs (0.895±0.339) μg/μl, P<0.01). All samples were determined for telomerase activity. It was confirmed that the activity of telomerase in normal bone marrow was lower than that of acute leukemia patients ((0.125±0.078) μg/μl vs (0.765±0.284) μg/μl, P<0.01). There was no significant difference of expression level of TRF1 protein between ALL and ANLL patients ((0.897±0.290) μg/μl vs (0.677±0.268) μg/μl, P>0.05). After chemotherapy, telomerase activity of patients with complete remission decreased ((0.393±0.125) μg/μl), but was still higher than that of normal ((0.125±0.078) μg/μl, P<0.01). Conclusion: The expression level of TRF1 protein has corre-lativity to the activity of telomerase (P<0.001).
Objective: To study the expression level of TRF1 (telomeric repeat binding factor 1) protein in human acute leukemia and relationship between expression level of TRF1 protein and telomerase, Methods: A quantitative Western±Blot technique was developed using anti±TRF1^33±277 monoclonal antibody and GST±TRFI purity protein as a standard to further determine the expression level of TRF1 protein in total proteins extracted from clinical specimens. Results: Bone marrow tissues of 20 acute leukemia patients were studied, 11 healthy donors' bone marrows were taken as a control. The expression level of TRF1 protein was significantly higher (P〈0.01) in normal bone marrow ((2.2174±0.462) μg/μl) than that of acute leukemia patients ((0.7544±0.343) μg/μl), But there was no remarkable difference between ALL and ANLL patients ((0.6184±0.285) μg/μl vs (0.8454±0.359) μg/μl, P〉0.05). After chemotherapy, TRFI expression level of patients with complete remission elevated ((0.7724±0.307)/μg/μl vs (1.6834±0,344)μg/μl, P〈0.01 ), but lower than that of normal ((2.2174±0.462)/μg/μl, P〈0.01). There was no significantly difference after chemotherapy ((0.7264±0.411) μg/μl vs (0.895±0.339) μg/μl,p〉0.05). TRF1 expression level of patients with complete remission is higher than that of patients without complete remission ((1,683±0.344)μg/μl vs (0.895±0.339)μg/μl P〈0.01). All samples were determined for telomerase activity. It was confirmed that the activity of telomerase in normal bone marrow was lower than that of acute leukemia patients ((0.125±0.078) μg/μl vs (0.765±0.284)μg/μl, P〈0.01). There was no significant difference of expression level ofTRF I protein between ALL and ANLL patients ((0.897±0.290) μg/μl vs (0.677±0.268) μg/μl, P〉0.05). After chemotherapy, telomerase activity of patients with complete remission decreased ((0.393±0.125) μg/μl), but was still higher than that of normal ((0.125±0.078) μg/μl, P〈0.01). Conclusion: The expression level of TRF1 protein has correlativity to the activity of telomerase (P〈0.001).
基金
Project(No.2002CB713704)supportedbytheNationalBasicRe-searchProgram(973)ofChina
关键词
急性白血病
TRF1蛋白
端粒末端转移酶
表达
Acute leukemia (AL), Human telomeric repeat binding factor protein 1 (TRFI), Monoclonal antibody, Expression level of TRF1 protein, Telomerase activity