肾综合征出血热患者血清及尿液中TNF与SIL－2R的动态研究

Study of tumor necrosis factor and soluble interleukin-2 receptor in serum and urine of hemorrhagic fever patients with renal syndrome

采用双抗体夹心ＥＬＩＳＡ法，对３０例肾综合征出血热（ＨＦＲＳ）患者９６份血清及９６份尿液中肿瘤坏死因子（ＴＮＦ）及可溶性白细胞介素２受体（ＳＩＬ－２Ｒ）水平进行了动态检测。结果显示：ＨＦＲＳ患者各期尿液及血清ＴＮＦ与ＳＩＬ－２Ｒ水平均高于正常对照组（Ｐ＜０．０１），且与临床病程密切相关，尿液与血清变化水平有密切的正相关性（γ＝０．５７５２，Ｐ＜０．００１；γ＝０．５４２７，Ｐ＜０．０１），尿液中含量与尿蛋白含量呈正相关（γ＝０．３４２５，Ｐ＜０．０１；γ＝０．４４８５，Ｐ＜０．０１），血、尿中ＴＮＦ与血、尿中ＳＩＬ－２Ｒ的变化呈正相关（γ＝０．６４２５，Ｐ＜０．０１，γ＝０．６５９６，Ｐ＜０．０１）。提示：ＴＮＦ及ＳＩＬ－２Ｒ是参与ＨＦＲＳ发病机理的重要介质。

Tumor necrosis factor(TNF)and soluble interleukin-2 receptor (SIL-2R) were assayed dynamically by double antibody sandwich ELISA method in 96 serum and 90 urine samples from 30 cases of hemorrhagic fever with renal syndrome.The results showed that TNF and SIL-2R levels in the urine and serum samples of the patients at different stages of hermorrhagic fever were all higher than those of healthy controls(P<0.01). It is closely related to the process of disease. A positive correlation between the levels in the urine and those in the serum was observed (γ=0.5752,P<0.01; γ0.5427,P<0.01).A positive correlation between levels of TNF and SIL-2R in the urine and levels of urinary protein was also observed (γ=0.3425,P<0.01;γ=0.4485,P<0.01). There are a positive relationship between changes of TNF and SIL-2R in urine and serum (γ=0.6425,P<0.01;γ=0.6596,P<0.01). The results showed that TNF and SIL-2R are important intervention in the pathologic process of hemorrhagic fever with renal syndrome.