东菱迪芙对抗肾小球基底膜肾炎兔血液流变学的影响

EFFECTS OF DF-521 ON HEMORRHEOLOGY OF ANTI-GBM NEPHRITIS IN RABBITS

目的:探讨抗肾小球基底膜(GBM)肾炎兔血液流变学的特点,观察东菱迪芙(DF-521)对抗GBM肾炎兔血液流变学的影响。方法:实验分3组:东菱迪芙治疗组(A组)、模型对照组(B组)和正常对照组(C组)。A组注射肾毒血清后6 h内开始给药,B组注射肾毒血清后给等量的生理盐水,C组仅给等量的生理盐水。观察一般情况,2周后采血测定全血黏度(ηb)、血浆黏度(ηp)、红细胞比容(HCT)、血沉(ESR)、红细胞聚集指数(EA I)、红细胞刚性指数(TK)、纤维蛋白原(F ig)含量。结果:B组较C组血流变学指标均显著增高(P<0.01);A组与B组比较,ηb、ηp、ESR、EA I、TK、F ig均明显降低(P<0.05)。结论:抗GBM肾炎兔存在血液流变学异常,东菱迪芙可能主要通过降低F ig,改善肾脏微循环与血液流变学。

Objective：To investigate the character of hemorrheology of anti-GBM nephritis in rabbits and the therapeutic effects of treatment of DF-521. Methods.Twenty-four New Zealand rabbit were randomly distributed into DF-521-treatment group（Group A）,untreated nephritic group（Group B） and normal group （Group C）. Autologous anti-GBM nephritis model was established with goat anti-rabbit GBM antibody （Ab） injecting into the rabbits in Group A and Group B. DF-521 was administered every day six hours after the injection of anti-GBM IgG. All the rabbits were sacrificed and the blood specimens were collected after 2 weeks, then ηb, ηp, HCT, ESR, EAI, TK and Concentration of fibrinogen were examined. Result ： In the Group B, all the hemorrheological indexes were increased significantly （ P 〈0.01） compared with Group C,and in the A group, ηb,ηp,ESR,EAI,TK（ P〈0.05）,Fig（ P 〈0.01） decreased significantly compared with Group B. Conclusion：The hemorrheologie behavior of rabbit anti-GBM nephritis is abnormal, and DF-521 can decrease the concentration of fibrnogen and improve the renal hemorrheology and microcirculation.