预吸氧对大鼠肝组织抗氧化损伤能力的影响

Effects of Preoxygenation on Hepatic Antioxidant Capacity in Rats

目的:探讨不同浓度预吸氧对肝脏抗氧化损伤能力的影响. 方法:24只成年雄性Wistar大鼠随机平均分成4组,Ⅰ组(对照组)不吸氧;Ⅱ～Ⅳ组分别吸入50%、75%和95%氧30 min后开腹取肝脏.测定肝组织丙二醛(MDA)含量、总超氧化物歧化酶(T-SOD)活力和总抗氧化能力( T-AOC).结果:Ⅰ～Ⅳ组T-SOD活力呈逐渐降低趋势,MDA含量呈逐渐升高趋势,两种指标Ⅳ组与Ⅰ组相比差异均有统计学意义(P<0.05);Ⅱ～Ⅳ组T-AOC均低于Ⅰ组(P<0.05).结论:50%以上浓度预吸氧可降低大鼠肝组织总抗氧化能力;95%浓度预吸氧有引起肝脏过氧化损伤的危险.

Objective： To investigate the effects of preoxygeaation on hepatic antioxidam capacity in rats. Method： Twenty-four Wistar rats （250-300g） were randondy divided into four groups （6 in each group）, group Ⅰ for control and group Ⅱ , Ⅲ and Ⅳ for preoxygenation. The rats in four groups were individually exposed to different concentration of oxygenation （21% ,50% ,75% and 95% ） for 30 rain and the total superoxide dismutase （T-SOD） , malondialdehyde （MDA） and total antioxidant capacity （T-AOC） in rat liver tissue were measured. Result： The T-SOD values decreased and the MDA values increased from group Ⅰ to grouop Ⅳ gradually , and there were significant differences in the two val- ues between grouop I and group IV （ P 〈 0.05 ）. The T-AOC values in group Ⅱ , Ⅲ and Ⅳ were higher significantly than that in group 1 （ P 〈 0.05 ）. Conclusion ： The preoxygenation with the concentration above 50% can decrease the antioxidant capacity and with the concentration of 95% may cause lipid perexidantion in rat liver.