摘要
目的:探讨不同浓度预吸氧对肝脏抗氧化损伤能力的影响. 方法:24只成年雄性Wistar大鼠随机平均分成4组,Ⅰ组(对照组)不吸氧;Ⅱ~Ⅳ组分别吸入50%、75%和95%氧30 min后开腹取肝脏.测定肝组织丙二醛(MDA)含量、总超氧化物歧化酶(T-SOD)活力和总抗氧化能力( T-AOC).结果:Ⅰ~Ⅳ组T-SOD活力呈逐渐降低趋势,MDA含量呈逐渐升高趋势,两种指标Ⅳ组与Ⅰ组相比差异均有统计学意义(P<0.05);Ⅱ~Ⅳ组T-AOC均低于Ⅰ组(P<0.05).结论:50%以上浓度预吸氧可降低大鼠肝组织总抗氧化能力;95%浓度预吸氧有引起肝脏过氧化损伤的危险.
Objective: To investigate the effects of preoxygeaation on hepatic antioxidam capacity in rats. Method: Twenty-four Wistar rats (250-300g) were randondy divided into four groups (6 in each group), group Ⅰ for control and group Ⅱ , Ⅲ and Ⅳ for preoxygenation. The rats in four groups were individually exposed to different concentration of oxygenation (21% ,50% ,75% and 95% ) for 30 rain and the total superoxide dismutase (T-SOD) , malondialdehyde (MDA) and total antioxidant capacity (T-AOC) in rat liver tissue were measured. Result: The T-SOD values decreased and the MDA values increased from group Ⅰ to grouop Ⅳ gradually , and there were significant differences in the two val- ues between grouop I and group IV ( P 〈 0.05 ). The T-AOC values in group Ⅱ , Ⅲ and Ⅳ were higher significantly than that in group 1 ( P 〈 0.05 ). Conclusion : The preoxygenation with the concentration above 50% can decrease the antioxidant capacity and with the concentration of 95% may cause lipid perexidantion in rat liver.
出处
《沈阳医学院学报》
2005年第4期212-213,共2页
Journal of Shenyang Medical College
关键词
预吸氧
肝脏
抗氧化
preoxygenation
liver
antioxidant