两种检测梅毒螺旋体抗体方法的比较

The Comparison between Two Methods to Test Antibody of Treponema Pallidum

目的:比较ELISA TP-IgG和快速血浆反应素试验(rap id p lasm a reagin c irc le card test,RPR)梅毒检测试剂的敏感性和特异性。方法:采用ELISA TP-IgG和RPR 2种试剂同时对56份临床疑似标本进行检测,结果阳性者采用梅毒螺旋体特异性抗体乳胶凝集试验(Toreponem a pallidum latex immuno assay,TPLA)方法进行验证。结果:ELISA TP-IgG检测率高于RPR,且与TPLA确证实验完全吻合。结论:ELISA TP方法在敏感性和特异性方面均好于RPR方法,是临床检测梅毒感染和筛选献血员的首选方法。

Objective： To compare the specificity and sensitivity of two methods for testing antibody of Trcponema pallidum（TP）. Methods： Fifty-six blood plasmas of patients were tested by ELISA TP-IgG and rapid plasma reagin circle card test （RPR）. Positive results were validated by Torcponema pallidum latex immuno assay （TPLA）. Results： The efficiency of ELISA TPdgG is higher than that of RPR. The test results of ELISA TP-IgG is accorded with that of TPLA. Conclusion： ELISA TP is more specific and sensitive than RPR, ELISA is more suitable for testing clinical TP infected persons and screening of blood donors.