鸡胚血液中原始生殖细胞的分离及其培养的研究

Study on Isdation and Culture of Primordial Germ Cells from Chick Embryos

从孵化４８～５５小时鸡胚中抽取血液，每只胚胎可获血液２～６μｌ左右。一步法离心分离原始生殖细胞，可使其浓度由０．１％以下提高到５０％以上。将多余血细胞用微量吸管移走后，加入添加１０％胎牛血清的ＴＣＭ—１９９做为培养基，３７．５℃，５％ＣＯ２，９５％空气，饱和湿度下培养，原始生殖细胞可成活２４小时左右。

Blood of 48hr- 55hr chick embryoes was drawn from heart or vitelline vein. Two-six microliters of blood could be drawn from each embryo.After one-step centrifugation, chick PGCs. could be concentrated from 0. 1 % to 50%,at the interface of b 6.3%(w/v)and 14.4 %(w/v)Ficou bilayer.Moved out the other blood cells with a micropipitte,added TCM - 199with 10% FCS to the PGCs as culture medium,in-vitro cultured in anincub ator with condition of 37. 5℃,5% CO2 and saturatinn,moisture,chick PGCs could survive for about 24hrs.