DNA杂交测序法

DNA Sequencing by hybridization

ＤＮＡ杂交测序法米志勇，柯，王丽霞（中国科学院发育生物学研究所植物发育分子生物学实验室，北京１０００８０）自从Ｓａｎｇｅｒ双脱氧末端终止法和Ｍａｘａｍ－Ｇｉｌｂｅｒ化学法问世以来，迄今已测定的ＤＮＡ核苷酸序列累计达１×１０８ｂｐ以上。但随着各种基因组...

UP to now,The DNA sequence data has accumulated over 1×108bp since the introduction of the chain-termination DNA sequencing and Maxam-Gilbert chemical methods. However,these two kinds of traditional methods will not widely meet with the need of the future DNA sequencing with the ev ery kinds of genomic programmes, no matter in efficiency or in cost. During 1990’s,Sequencing by hy bridization-SBH was reported by several labs. Through hybridization between marked single strand DNA template and specific length random oligo-nucleotides,We can obtain DNA sequence data quick ly.