摘要
DNA杂交测序法米志勇,柯,王丽霞(中国科学院发育生物学研究所植物发育分子生物学实验室,北京100080)自从Sanger双脱氧末端终止法和Maxam-Gilber化学法问世以来,迄今已测定的DNA核苷酸序列累计达1×108bp以上。但随着各种基因组...
UP to now,The DNA sequence data has accumulated over 1×108bp since the introduction of the chain-termination DNA sequencing and Maxam-Gilbert chemical methods. However,these two kinds of traditional methods will not widely meet with the need of the future DNA sequencing with the ev ery kinds of genomic programmes, no matter in efficiency or in cost. During 1990’s,Sequencing by hy bridization-SBH was reported by several labs. Through hybridization between marked single strand DNA template and specific length random oligo-nucleotides,We can obtain DNA sequence data quick ly.
出处
《生物工程进展》
CSCD
1996年第3期54-56,共3页
Progress in Biotechnology