黄芪总苷对脾虚大鼠壁细胞胃泌素受体作用的研究

Effect of Astragalosides on Gastrin Receptor in Gastric Parietal Cells of Rats with Spleen Deficiency

观察黄芪总苷(AST)对脾虚大鼠胃壁细胞胃泌素受体结合位点数变化的调控作用。【方法】SD大鼠采用胃饲大黄液(10g／kg)方法复制脾虚模型;采用Lewin胃袋法及Percoll密度梯度纯化法分离与纯化大鼠胃粘膜壁细胞;按10、 20、30 g／L设AST低、中、高剂量组,在每2mL脾虚模型大鼠胃壁细胞悬液中加入AST20μL体外孵育,并设正常对照组与模型组;采用放射配基结合法检测壁细胞胃泌素受体结合位点数。【结果】模型组壁细胞胃泌素受体结合位点数下降,与正常对照组比较具有显著性差异(P<0．05);AST高剂量组可上调胃泌素受体结合位点数,与模型组比较具有显著性差异 (P<0．05)。【结论】黄芪总苷可增加脾虚模型大鼠因脾虚而降低的胃壁细胞胃泌素受体结合位点数,可能是黄芪发挥益气健脾作用的主要有效成分(部位)。

[ Objective ] To observe the regulatory effect of astragalosides （AST） on the binding capacity of gastrin receptor in gastric parietal cells （GPC） of rats with spleen deficiency. [ Methods ] SD rats were given gastric gavage of Radix et Rhizoma Rhei （Dahuang） decoction 10 g/kg to induce spleen deficiency. Gastric parietal cells were isolated and purified by Lewin gastric sac method and Percoll density gradient separation method. 2 mL suspension of GPC from rat models was incubated in vitro with AST 20μL at the concentrations of 10, 20 and 30 g/L respectively. Suspension of GPC from rat models incubated with phosphate buffered saline （PBS） served as the model group and suspension of GPC from the normal rats incubated with PBS as the normal group. The binding capacity of gastrin receptor in GPC was detected by radioligand receptor assay. [ Results ] The binding sites were 262.29 ± 60.80 in the model group, much lower than 443.93 ± 133.58 in the normal group （ P 〈 0.05）. The binding sites were 665.91 ± 522.28 in high-dose AST group, decreasing as compared with those in the normal group （ P 〈 0.05）. [ Conclusion ] AST can increase the binding capacity of gastrin receptor Radix Astragali （ Huangqi ） which in GPC of rats with spleen deficiency, and this may be the main active compounds has the actions of invigorating qi and strengthening spleen.