摘要
以食用仙人掌的幼嫩肉质茎和紫糯玉米的幼嫩叶片为试验材料,采用CTAB法提取DNA,用紫外分光光度计检测DNA的浓度和琼脂糖凝胶电泳检测DNA的质量。结果表明,采用CTAB法可以从食用仙人掌中提取到一定质量和浓度的DNA,但是在操作过程中利用Tris-饱和酚对其进行纯化处理是非常必要的。
DNA of Tender fleshy stem of Opuntia Ficus-indica and tender leaf of purple glutinous maize was extracted by method of CTAB. Concentration of DNA was measured by UV spectrophotometer, and quality of DNA was examined by agarose gel. Result suggested that better DNA of Opuntia Ficusindica was getted by method of CTAB, but it is necessary to purify by used Tris-Phenol.
出处
《河南农业科学》
CSCD
北大核心
2006年第1期84-86,共3页
Journal of Henan Agricultural Sciences
基金
国家"十五"科技攻关项目(2001BA302B)
