几种免疫增强剂对中华鳖红细胞数量及免疫功能的影响

Effects of immuno-stimunants on the number of erythrocytes and immune functions of Trionyx sinensis

在基础饲料中添加2.0μg/g亚硒酸钠、0.5μg/g硒酵母、0.3%β-葡聚糖和0.25%果糖,投喂100 d后,测定中华鳖红细胞数量和四个免疫指标的变化。主要结果为:(1)在红细胞数量方面,亚硒酸钠组、果糖组、硒酵母组显著高于β-葡聚糖组及对照组(P<0.05);(2)在红细胞C3b受体花环率和T淋巴细胞活性E花环率方面,四个试验组显著高于对照组(P<0.05);(3)在红细胞天然免疫粘附肿瘤细胞花环率上,仅亚硒酸钠组显著高于对照组(P<0.05);(4)在白细胞吞噬活性上,亚硒酸钠组显著高于β-葡聚糖及对照组(P<0.05),硒酵母组、果糖组显著高于对照组(P<0.05),但β-葡聚糖组与对照组间差异不显著(P>0.05);(5)红细胞C3b花环率与T淋巴细胞活性E花环率间存在明显的正相关(P<0.01),表明中华鳖红细胞具有免疫调节作用。以上结果表明,在饲料中添加不同的免疫增强剂,能提高中华鳖血液中红细胞含量,增进免疫功能,从而提高对疾病的抵抗力。

The number of erythrocytes and indexes of immune function of Trionyx sinensis, which was fed with basal formulated diet supplemented with 2.0 μg/g Na2SeO3, 0.5μg/g selenoyeast, 0.3% betaglucan and 0.25% fructooligosaccharides for 100 days, were measured. The major results were： （1）On the number of erythmcytes, Na2SeO3, fructooligosaccharides and selenoyeast group were significantly higher than that of betaglucan and control group （P 〈 0.05）. （2） On the percentage of erythrocyte C3b rosette and of erythmcyte active rosette （EAR） of T lymphocyte, the four test groups were all significantly higher than that of control group （ P 〈 0.05）. （3） On the percentage of erythmcyte native immune adhering tumor cell rosette, only Na2SeO3 group was significantly higher than that of control group （ P 〈 0.05）. （4）On the activity of leukocyte phagocyte, Na2SeO3 group was significantly higher than that of betaglucan and control groups （ P 〈 0.05）, also selenoyeast and fructooligosaccharides groups were significantly higher than that of control group （ P 〈 0.05）, but there was no significant difference between betaglucan and control group （P 〉 0.05）. （5） There was a remarkable correlation between the percentage of erythrocyte C3b rosette and EaR of T lymphocyte （ P 〈 0.01 ）, indicating immuno-modulation of erythrocyte of T. sinensis. All the results showed that immuno-stimunants supplemented in the basal formulated diet significantly elevated the number of erythrocytes and promoted the immune functions of T. sinensis, which might resuh in high resistance to diseases.