红鳍东方鲀微卫星DNA多态性初步分析

Analyzing the polymorphisms of Takifugu rubripes with microsatellite

用红鳍东方(Takifugu rubripes)微卫星标记对两个不同群体红鳍东方的DNA多态性进行分析。经20对引物的扩增,得到每个个体的微卫星片段,其中16对引物能扩增出较好的谱带。用2%的琼脂糖凝胶电泳检测微卫星标记的PCR扩增产物,并对扩增结果进行了统计分析。计算得到两个群体的平均等位基因数分别为1.875 0和2.437 5;多态信息含量(PIC)平均值分别为0.275 1和0.379 4;平均杂合度观测值分别为0.025和0.200。统计结果初步表明两个不同群体平均杂合度较低,遗传多态性不高。

Two populations of Takifugu rubripes domesticated in different places were, analyzed with the Takifugu frbripes microsatellite marker and the pattern of each sample was obtained. Altogether 20 primers were used in this experiment,of which 16 primers were assessed for genetic diversity for 10 individuals. The PCR products were electrophoresed by 2% agarose gel, and the data like allelic frequencies and analyzed by statistic method. The results indicated：mean number of alleles were 1. 875 0 and 2.437 5 , PIC value（polymorphism information content） were 0. 275 1 and 0. 379 4 and the observed heterozygosity values over all loci were 0.025 and 0. 200. All of these indices indicated that genetic diversity of Takifugu rubripes was not inspiring.