人肝癌细胞N-ras基因RNA干涉有效靶点的确定

Ascertainment of effective RNAi sequences of N-ras in humen hepatocellular carcinoma cells

目的寻找人肝癌细胞N-ras基因的RNA干涉有效靶点。方法利用计算机网络资源及Oligo6.0、BlastResearch等生物学软件在N-ras基因编码区寻找RNAi有效靶点,并设计用于体内转录形成以U6启动子的siRNA发夹结构的DNA。结果成功筛选出RNA干涉的有效序列14个,并设计出siRNA发夹结构的DNA。结论这种对人肝癌细胞N-ras基因的RNAi有效靶点的筛选为进一步研究奠定了理论基础。其工作的开展将在RNAi治疗、肝癌N-ras基因功能研究、新药开发等方面发挥重要作用。

Objective To ascertain the effective RNAi sequences of N-ras in humen hepatocellular carcinoma cell.Methods Using computer network resouees and Oligo6.0 and Blast Research software can gain the effective RNAi sequences of N-ras coding region. The hairpin DNA was designed for forming siRNA in vivo transcription.Results To screen fourteen effective RNAi sequences successfully and the hairpin DNA.Conclusion The effective RNAi sequences of N - ras has been successfully acquired, which may provide the theory foundation for the further experimental researches. The researches would make the important effect on the RNAi treatment,the function study of N - ras in humen hepatocellular carcinoma cells and the new drug design for hepatocarcinoma.