摘要
目的:优选出蜂胶环糊精最佳包合工艺并考察其对小鼠辐射损伤的保护作用。方法:以总黄酮含量为指标,选用L9(34)正交实验表进行实验,优选β-环糊精包合蜂胶的最佳工艺;以该蜂胶环糊精包合物为实验药物,以60Coγ射线照射造成小鼠辐射损伤,观察小鼠30天内存活时间、并进行常规白细胞计数及硫代巴比妥酸法测定肝脏中脂质过氧化物含量。结果:β-环糊精包合蜂胶的最佳工艺条件是:蜂胶:-βCD比例为1∶2,乙醇浓度为95%,水浴温度为50℃;与模型组比较,蜂胶能显著延长小鼠辐射后30d内存活时间(P<0.01)、提高小鼠外周血白细胞数(P<0.01)、降低肝脏脂质过氧化物含量(P<0.01)。结论:蜂胶对辐射损伤具有保护作用。
Objective：To study the optimum technology of preparation propolis wrapped with β-cyclodextrin （β- CD） and its protective effect on radiation injury in rats. Methods： Using orthogonal design method to optimize the technology of preparation propolis wrapped with β-CD, and the amount of flavone was used as a parameter evaluating outcome. Survival duration in 30 days, WBC and MDA were measured in rats after exposed to γ-radiation of 7 Gy. Results：The optimum conditions is：ratio of Propolis to β-cyclodextrin is 1 to 2, bathing temperature at 50 degrees Celsius and 95 percent alcohol;compared with the model group, survival duration in 30 days and WBC were increased significantly （P 〈0.01 ）,the amount of MDA was decreased markedly （P 〈 0.01 ） in propolis treated group. Conclusion： propolis showed the effective of protecting radiation injury.
出处
《中国实验方剂学杂志》
CAS
2006年第1期39-41,共3页
Chinese Journal of Experimental Traditional Medical Formulae
关键词
蜂胶
总黄酮
Β-环糊精
辐射损伤
propolis
flavone
β-cyclodextrin
radiation injury
