摘要
目的:了解清热合剂对呼吸道合胞病毒(RSV)的抑制作用。方法:采用细胞培养技术,以双黄连为阳性对照药,观察清热合剂在非洲绿猴肾细胞(VERO)和咽喉癌上皮细胞(HEP-2)细胞中对RSV的抑制作用。结果:在VERO细胞中,清热合剂半数中毒浓度(TD50)为31.62mg/mL,抗合胞病毒的半数有效浓度(IC50)为0.50mg/mL,治疗指数(TI)为63.24;在HEP-2细胞中,TD50为32.10mg/mL,抗合胞病毒的IC50为0.54mg/mL,TI为59.44。且对RSV抑制作用存在明显的量效反应关系(P<0.01)。结论:清热合剂在VERO和HEP-2细胞中对RSV有明显抑制作用。
Objective：To observe the anti-respiratory syncyntial virus （RSV） effect of Qing Re He Ji （QRHJ）. Methods：The anti-RSV effect of QRHJ was observed by means of the technique of VERO and HEP-2 cell culture using Shuang Huang Lian as a positive control. Results： In VERO and HEP-2 cell culture, QRHJ was a potential inhibitor of RSV in concentration-dependent manner（ P 〈 0.01 ）. The TD50 was 31.62mg/mL, the ICso was 0.50mg/mL and TI was 63.24 in VERO cell culture. The TDso was 32.10mg/mL, the IC50 was 0.54mg/mL and TI was 59.44 in HEP-2 cell culture. Conclusion：ORHJ has a sianificant anti-RSV effect in VERO and HEP-2 cell culture.
出处
《中国实验方剂学杂志》
CAS
2006年第1期53-55,共3页
Chinese Journal of Experimental Traditional Medical Formulae
关键词
呼吸道合胞病毒
抗病毒作用
清热合剂
respiratory syncyntial vires
anti-viral effect
Qing Re He Ji
