平阳霉素局部注射治疗鼻息肉病机制探讨

Study on the treatment of nasal polyposis with bleomycin A_5 local injection

目的:观察平阳霉素局部注射治疗对鼻息肉病息肉组织中嗜酸粒细胞凋亡、转化生长因子(TGF-β1))表达的影响,探讨其治疗鼻息肉病的机制。方法:对19例平阳霉素局部注射治疗前后的鼻息肉病鼻息肉组织进行免疫组织化学染色检测TGF-β1表达;应用原位杂交法(TUNEL)检测凋亡细胞。结果:嗜酸粒细胞凋亡指数治疗前为(20.53±7.66)%,治疗后为(44.47±8.97)%,治疗前后差异有统计学意义(P<0.01);治疗后嗜酸粒细胞TGF-β1阳性表达率(74.74±5.22)%低于治疗前(85.18±8.07)%,治疗后TGF-β1在鼻息肉组织基质中的表达低于治疗前,差异均有统计学意义(P<0.05)。结论:平阳霉素可通过促进嗜酸粒细胞凋亡及减少TGF-β1在嗜酸粒细胞和基质中的表达治疗鼻息肉病。

Objective：To study the treatment mechanism of nasal polyposis by locally injecting Bleomycin A5. Method.. The specimens from nineteen cases with nasal polyposis obtained before and after treatment with BleomyT cin A5 were studied with immunohistoehemieal technique and terminal deoxynucleotidyl transferasemediated dUTP nick end labeling（TUNEL）. Result： The apoptosis index（AI） of eosinophil was （20.53± 7.66）% before treatment and （44.47±8.97）% after treatment. There was significantly difference between them （ P〈0.01）. The rate of TGF-β1 positive expression in eosinophil after treatment（74.74±5.22）% was significantly lower than that before treatment （85.18± 8. 07）% （ P 〈0.05）. The expression of TGF-β1 in matrix was also decreased（ P 〈0.05）. Conclusion：Bleomycin A5 could cure nasal polyposis by promoting eosinophil apoptosis and reducing the expression of TGF-β1.